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Protein-based molecular contrast optical coherence tomography with phytochrome as the contrast agent

Yang, Changhuei and Choma, Michael A. and Lamb, Laura E. and Simon, John D. and Izatt, Joseph A. (2004) Protein-based molecular contrast optical coherence tomography with phytochrome as the contrast agent. Optics Letters, 29 (12). pp. 1396-1398. ISSN 0146-9592. http://resolver.caltech.edu/CaltechAUTHORS:YANol04b

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Abstract

We report the use of phytochrome A (phyA), a plant protein that can reversibly switch between two states with different absorption maxima (at 660 and 730 nm), as a contrast agent for molecular contrast optical coherence tomography (MCOCT). Our MCOCT scheme builds up a difference image revealing the distribution of phyA within a target sample from pairs of consecutive OCT A-scans acquired at a probe wavelength of 750 nm, both with and without additional illumination of the target sample with 660-nm light. We demonstrate molecular imaging with this new MCOCT modality in a target sample containing a mixture of 0.2% Intralipid and 83 µM of phyA.


Item Type:Article
Additional Information:© 2004 Optical Society of America Received January 2, 2004 This research was supported by National Institutes of Health grant EB000243.
Subject Keywords:Optical coherence tomography; Medical and biological imaging; Molecular spectroscopy; Photochemistry
Record Number:CaltechAUTHORS:YANol04b
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:YANol04b
Alternative URL:http://www.opticsinfobase.org/abstract.cfm?URI=ol-29-12-1396
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:3323
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:30 May 2006
Last Modified:26 Dec 2012 08:53

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