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Electron tunneling through mutant azurins on mixed SAM gold electrodes

Yokoyama, K. and Nakamura, N. and Ohno, H. and Winkler, J. R. and Richards, J. H. and Gray, H. B. (2010) Electron tunneling through mutant azurins on mixed SAM gold electrodes. In: International Chemical Congress of Pacific Basin Societies (Pacifichem 2010), December 15-20, 2010, Honolulu, HI. http://resolver.caltech.edu/CaltechAUTHORS:20120816-072045664

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Abstract

We are investigating interfacial electron transfer rates of P. aeruginosa azurin and its mutants using electrochem. at 1:1 CH3(CH2)nSH:HO(CH2)nSH mixed-SAM gold electrodes. We have examd. interfacial electron transfer rates of mutant azurins in which asparagine-47 was replaced by alanine, aspartic acid, lysine, arginine, leucine, threonine, serine, and glutamine. The N47D mutant on a mixed SAM exhibited a well-defined electrochem. response; N47T and N47S gave a weak signal; but the other 5 mutants showed no response. It is likely that the N47 side-chain carbonyl interacts with the mixed SAM surface, providing a very favorable electron tunneling pathway to the copper via an N47-C112 hydrogen bond. We also have examd. interfacial electron transfer rates of M121X mutant azurins. The ET rates for M121E are 2 orders of magnitude smaller; those for M121Q are almost 1 order of magnitude smaller; and those for the M121L protein are similar to those of wild-type azurin at pH 7. In addn., we have found that type zero azurins have higher ET rates than those of type 2 proteins (rates for C112D are 3 orders of magnitude smaller; those for type zero C112D/M121X (X=L and I) are 2 orders of magnitude smaller than wild-type at pH 7).


Item Type:Conference or Workshop Item (Paper)
Additional Information:© 2012 American Chemical Society.
Record Number:CaltechAUTHORS:20120816-072045664
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20120816-072045664
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:33247
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:16 Aug 2012 18:49
Last Modified:16 Aug 2012 18:49

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