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Spectral-domain phase microscopy

Choma, Michael A. and Ellerbee, Audrey K. and Yang, Changhuei and Creazzo, Tony L. and Izatt, Joseph A. (2005) Spectral-domain phase microscopy. Optics Letters, 30 (10). pp. 1162-1164. ISSN 0146-9592. http://resolver.caltech.edu/CaltechAUTHORS:CHOol05c

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Abstract

Broadband interferometry is an attractive technique for the detection of cellular motions because it provides depth-resolved phase information via coherence gating. We present a phase-sensitive technique called spectral-domain phase microscopy (SDPM). SDPM is a functional extension of spectral-domain optical coherence tomography that allows for the detection of nanometer-scale motions in living cells. The sensitivity of the technique is demonstrated, and its calibration is verified. A shot-noise limit to the displacement sensitivity of this technique is derived. Measurement of cellular dynamics was performed on spontaneously beating cardiomyocytes isolated from chick embryos.


Item Type:Article
Additional Information:© 2005 Optical Society of America Received October 8, 2004 This work was supported by National Institutes of Health grants R24-EB000243 and R21-RR019769 to J. A. Izatt and grants R01 HL715015 and P01 HL36059 to T. L. Creazzo.
Subject Keywords:Noise in imaging systems; Optical coherence tomography; Interferometry; Interference microscopy
Record Number:CaltechAUTHORS:CHOol05c
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:CHOol05c
Alternative URL:http://www.opticsinfobase.org/abstract.cfm?URI=ol-30-10-1162
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:3332
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:31 May 2006
Last Modified:08 Dec 2014 23:22

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