Choma, Michael A. and Ellerbee, Audrey K. and Yang, Changhuei and Creazzo, Tony L. and Izatt, Joseph A. (2005) Spectral-domain phase microscopy. Optics Letters, 30 (10). pp. 1162-1164. ISSN 0146-9592 http://resolver.caltech.edu/CaltechAUTHORS:CHOol05c
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Broadband interferometry is an attractive technique for the detection of cellular motions because it provides depth-resolved phase information via coherence gating. We present a phase-sensitive technique called spectral-domain phase microscopy (SDPM). SDPM is a functional extension of spectral-domain optical coherence tomography that allows for the detection of nanometer-scale motions in living cells. The sensitivity of the technique is demonstrated, and its calibration is verified. A shot-noise limit to the displacement sensitivity of this technique is derived. Measurement of cellular dynamics was performed on spontaneously beating cardiomyocytes isolated from chick embryos.
|Additional Information:||© 2005 Optical Society of America Received October 8, 2004 This work was supported by National Institutes of Health grants R24-EB000243 and R21-RR019769 to J. A. Izatt and grants R01 HL715015 and P01 HL36059 to T. L. Creazzo.|
|Subject Keywords:||Noise in imaging systems; Optical coherence tomography; Interferometry; Interference microscopy|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Archive Administrator|
|Deposited On:||31 May 2006|
|Last Modified:||26 Dec 2012 08:53|
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