Stampfer, Martha and Baltimore, David and Huang, Alice S. (1969) Ribonucleic acid synthesis of vesicular stomatitis virus. I. Species of Ribonucleic Acid Found in Chinese Hamster Ovary Cells Infected with Plaque-forming and Defective Particles. Journal of Virology, 4 (2). pp. 154-161. ISSN 0022-538X. http://resolver.caltech.edu/CaltechAUTHORS:STAjvir69
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Plaque-forming B particles of vesicular stomatitis virus (VSV) induce the synthesis of virus-specific ribonucleic acid (RNA) in Chinese hamster ovary cells, whereas defective T particles do not. Infection with low input multiplicities of B results in the formation of four species of RNA. During infection with high multiplicities, RNA synthesis begins with mainly these four species of RNA but gradually shifts to a new pattern of RNA synthesis involving five other species of RNA. The change can also be induced by superinfection with T at 2.5 hr after infection with a low multiplicity of B. T added at the same time as B prevents virtually all RNA synthesis. Synthesis of the first group of RNA species correlates with the formation of B particles, whereas synthesis of the second group correlates with the formation of T particles. The various species of RNA formed after infection with VSV particles include single-stranded RNA, a completely double-stranded RNA, and RNA with partially double-stranded regions. These observations begin to establish a molecular basis for understanding the ability of T particles to interfere with the growth of B particles.
|Additional Information:||Copyright © 1969 by the American Society for Microbiology. Received for publication 9 June 1969. This work was supported by Public Health Service grant AI-08388 from the National Institute of Allergy and Infectious Diseases and by American Cancer Society Research Grant E-512. M.S. is a recipient of a predoctoral fellowship from the National Science Foundation, D.B. received a Faculty Research Award from the American Cancer Society, and A.S.H. was a recipient of a postdoctoral fellowship from the Public Health Service (F2-AI-35,110). We thank Frensch Niegermeier for his critical reading of the manuscript, and Dianne Jack and Donna Smoler for technical assistance.|
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|Deposited By:||Tony Diaz|
|Deposited On:||01 Jun 2006|
|Last Modified:||03 Dec 2015 21:49|
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