CaltechAUTHORS
  A Caltech Library Service

Ribonucleic acid synthesis of vesicular stomatitis virus. I. Species of Ribonucleic Acid Found in Chinese Hamster Ovary Cells Infected with Plaque-forming and Defective Particles

Stampfer, Martha and Baltimore, David and Huang, Alice S. (1969) Ribonucleic acid synthesis of vesicular stomatitis virus. I. Species of Ribonucleic Acid Found in Chinese Hamster Ovary Cells Infected with Plaque-forming and Defective Particles. Journal of Virology, 4 (2). pp. 154-161. ISSN 0022-538X. http://resolver.caltech.edu/CaltechAUTHORS:STAjvir69

[img]
Preview
PDF
See Usage Policy.

1144Kb

Use this Persistent URL to link to this item: http://resolver.caltech.edu/CaltechAUTHORS:STAjvir69

Abstract

Plaque-forming B particles of vesicular stomatitis virus (VSV) induce the synthesis of virus-specific ribonucleic acid (RNA) in Chinese hamster ovary cells, whereas defective T particles do not. Infection with low input multiplicities of B results in the formation of four species of RNA. During infection with high multiplicities, RNA synthesis begins with mainly these four species of RNA but gradually shifts to a new pattern of RNA synthesis involving five other species of RNA. The change can also be induced by superinfection with T at 2.5 hr after infection with a low multiplicity of B. T added at the same time as B prevents virtually all RNA synthesis. Synthesis of the first group of RNA species correlates with the formation of B particles, whereas synthesis of the second group correlates with the formation of T particles. The various species of RNA formed after infection with VSV particles include single-stranded RNA, a completely double-stranded RNA, and RNA with partially double-stranded regions. These observations begin to establish a molecular basis for understanding the ability of T particles to interfere with the growth of B particles.


Item Type:Article
Additional Information:Copyright © 1969 by the American Society for Microbiology. Received for publication 9 June 1969. This work was supported by Public Health Service grant AI-08388 from the National Institute of Allergy and Infectious Diseases and by American Cancer Society Research Grant E-512. M.S. is a recipient of a predoctoral fellowship from the National Science Foundation, D.B. received a Faculty Research Award from the American Cancer Society, and A.S.H. was a recipient of a postdoctoral fellowship from the Public Health Service (F2-AI-35,110). We thank Frensch Niegermeier for his critical reading of the manuscript, and Dianne Jack and Donna Smoler for technical assistance.
Record Number:CaltechAUTHORS:STAjvir69
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:STAjvir69
Alternative URL:http://jvi.asm.org/cgi/content/abstract/4/2/154
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:3343
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:01 Jun 2006
Last Modified:26 Dec 2012 08:53

Repository Staff Only: item control page