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Genetic Expression in Heterozygous Replicative Form Molecules of {varphi}X174

Merriam, V. and Dumas, L. B. and Sinsheimer, R. L. (1971) Genetic Expression in Heterozygous Replicative Form Molecules of {varphi}X174. Journal of Virology, 7 (5). pp. 603-611. ISSN 0022-538X. http://resolver.caltech.edu/CaltechAUTHORS:MERjvir71

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Abstract

Heterozygous replicative form molecules of bacteriophage {varphi}X174 deoxyribonucleic acid (DNA) have been constructed in vitro. These are composed of viral strands extracted from purified preparations of phage bearing ts mutations and complementary strands of either half length or full length synthesized with purified DNA polymerase, in vitro, on DNA from am3 phage. In infections with such heterozygous DNA, involving mutations in each of four different cistrons, phage with the genotype of the complementary strand comprised 1 to 20% of the total phage produced by a spheroplast population. From single-burst analysis of the progeny from DNA heterozygous in one cistron (B), it appears that those phage with the genotype of the complementary strand arise as major components in a small proportion of the infected cells rather than comprising a minor component in most cells. The implications of such a pattern of expression are discussed with respect to mechanisms of phage DNA synthesis.


Item Type:Article
Additional Information:Copyright © 1971 American Society for Microbiology. Received for publication 4 January 1971 This research was supported by Public Health Service grant GM 13554 fromi the National Institute of General Medical Sciences. One of us (V.M.) wishes to thank Dan Ray for the use of the facilities of UCLA for the comiipletion of some of these experiments.
Record Number:CaltechAUTHORS:MERjvir71
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:MERjvir71
Alternative URL:http://jvi.asm.org/cgi/content/abstract/7/5/603
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:3392
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:05 Jun 2006
Last Modified:26 Dec 2012 08:54

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