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Nature of {varphi}X174 Linear DNA from a DNA Ligase-Defective Host

Miller, Lois K. and Sinsheimer, Robert L. (1974) Nature of {varphi}X174 Linear DNA from a DNA Ligase-Defective Host. Journal of Virology, 14 (6). pp. 1503-1514. ISSN 0022-538X.

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Linear DNAs have been prepared from {varphi}X phage and from {varphi}X RF II (double-stranded circular form of {varphi}X DNA, formed during infection and nicked in one or both strands) molecules derived from infection at the restrictive temperature of Escherichia coli ts7, a host mutant with a temperature-sensitive DNA ligase activity. The linear DNA from these phages can be circularized by annealing with fragments of {varphi}X RF DNA produced by the Haemophilus influenzae restriction nuclease. The circularization experiment indicated that the site of breakage of the linear phage DNAs is not unique nor confined to a particular region of the genome. These linear DNAs were less than 0.1% as infective as circular phage DNA. The linear, positive strand of late RF II DNA, however, is uniquely nicked in the region of the {varphi}X genome corresponding to cistron A. Although a low level of infectivity is associated with the linear DNA derived from late RF II, this infectivity appears to be a result of the association of linear positive and linear negative strands during the infectivity assay.

Item Type:Article
Additional Information:Copyright © 1974 American Society for Microbiology. Received for publication 15 July 1974 This research was supported by Public Health Service grant no. GM-13554 from the National Institute of General Medical Sciences. One of us (L.K.M.) was supported by postdoctoral research fellowship no. PF-803 from the American Cancer Society.
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Deposited On:05 Jun 2006
Last Modified:26 Dec 2012 08:54

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