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Sphingosine Phosphate Lyase Expression Is Essential for Normal Development in Caenorhabditis elegans

Mendel, Jane and Heinecke, Karie and Fyrst, Henrik and Saba, Julie D. (2003) Sphingosine Phosphate Lyase Expression Is Essential for Normal Development in Caenorhabditis elegans. Journal of Biological Chemistry, 278 (25). pp. 22341-22349. ISSN 0021-9258. http://resolver.caltech.edu/CaltechAUTHORS:MENjbc03

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Abstract

Sphingolipids are ubiquitous membrane constituents whose metabolites function as signaling molecules in eukaryotic cells. Sphingosine 1-phosphate, a key sphingolipid second messenger, regulates proliferation, motility, invasiveness, and programmed cell death. These effects of sphingosine 1-phosphate and similar phosphorylated sphingoid bases have been observed in organisms as diverse as yeast and humans. Intracellular levels of sphingosine 1-phosphate are tightly regulated by the actions of sphingosine kinase, which is responsible for its synthesis and sphingosine-1-phosphate phosphatase and sphingosine phosphate lyase, the two enzymes responsible for its catabolism. In this study, we describe the cloning of the Caenorhabditis elegans sphingosine phosphate lyase gene along with its functional expression in Saccharomyces cerevisiae. Promoter analysis indicates tissue-specific and developmental regulation of sphingosine phosphate lyase gene expression. Inhibition of C. elegans sphingosine phosphate lyase expression by RNA interference causes accumulation of phosphorylated and unphosphorylated long-chain bases and leads to poor feeding, delayed growth, reproductive abnormalities, and intestinal damage similar to the effects seen with exposure to Bacillus thuringiensis toxin. Our results show that sphingosine phosphate lyase is an essential gene in C. elegans and suggest that the sphingolipid degradative pathway plays a conserved role in regulating animal development.


Item Type:Article
Additional Information:Copyright © 2003 by the American Society for Biochemistry and Molecular Biology. Received for publication, March 20, 2003. Originally published In Press as doi:10.1074/jbc.M302857200 on April 7, 2003 We thank Betsy Lathrop for expert administrative assistance and Paul Sternberg for providing laboratory space. This work was supported by National Institutes of Health Grant 1R01CA77528 and by the Seaver Foundation. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Record Number:CaltechAUTHORS:MENjbc03
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:MENjbc03
Alternative URL:http://dx.doi.org/10.1074/jbc.M302857200
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:3396
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:05 Jun 2006
Last Modified:26 Dec 2012 08:54

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