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A long-term in vitro silicon-based microelectrode-neuron connection

Regehr, Wade G. and Pine, Jerome and Rutledge, David B. (1988) A long-term in vitro silicon-based microelectrode-neuron connection. IEEE Transactions on Biomedical Engineering, 35 (12). pp. 1023-1032. ISSN 0018-9294. http://resolver.caltech.edu/CaltechAUTHORS:REGieeetbe88

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Abstract

A novel method for long-term recording and simulation applicable to cultured neurons has been developed. Silicon-based microelectrodes have been fabricated using integrated-circuit technology and micromachining. The chronic connection is made by positioning the tip of the `diving-board electrode' into contact with the top of the cell body. The electrode support structure is then glued to the bottom of the culture dish. Two-way electrical connections to Helisoma B19 neurons have been maintained for up to four days. This capability makes it possible to conduct experiments that are not practical using conventional techniques.


Item Type:Article
Additional Information:© Copyright 2006 IEEE. Reprinted with permission. Manuscript received February 29, 1988; revised June 22, 1988. This work was supported by NSF Grant BNS8603713. The authors are grateful to Dr. S . B. Kater for supplying Helisoma neurons and for making his laboratory available for experiments, and to Dr. C. Cohan for helping with the Helisoma physiology.
Subject Keywords:2-way electrical connections; Helisoma B19 neurons; Si-based microelectrode neuron connection; diving-board electrode; electrode support structure; integrated-circuit technology; micromachining; neuroscience method
Record Number:CaltechAUTHORS:REGieeetbe88
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:REGieeetbe88
Alternative URL:http://dx.doi.org/10.1109/10.8687
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:3432
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:06 Jun 2006
Last Modified:26 Dec 2012 08:54

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