Abelson, John and Trotta, Christopher R. and Li, Hong (1998) tRNA splicing. Journal of Biological Chemistry, 273 (21). pp. 12685-12688. ISSN 0021-9258. http://resolver.caltech.edu/CaltechAUTHORS:ABEjbc98
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Introns interrupt the continuity of many eukaryal genes, and therefore their removal by splicing is a crucial step in gene expression. Interestingly, even within Eukarya there are at least four splicing mechanisms. mRNA splicing in the nucleus takes place in two phosphotransfer reactions on a complex and dynamic machine, the spliceosome. This reaction is related in mechanism to the two self-splicing mechanisms for Group 1 and Group 2 introns. In fact the Group 2 introns are spliced by an identical mechanism to mRNA splicing, although there is no general requirement for either proteins or co-factors. Thus it seems likely that the Group 2 and nuclear mRNA splicing reactions have diverged from a common ancestor. tRNA genes are also interrupted by introns, but here the splicing mechanism is quite different because it is catalyzed by three enzymes, all proteins and with an intrinsic requirement for ATP hydrolysis. tRNA splicing occurs in all three major lines of descent, the Bacteria, the Archaea, and the Eukarya. In bacteria the introns are self-splicing (1-3). Until recently it was thought that the mechanisms of tRNA splicing in Eukarya and Archaea were unrelated as well. In the past year, however, it has been found that the first enzyme in the tRNA splicing pathway, the tRNA endonuclease, has been conserved in evolution since the divergence of the Eukarya and the Archaea. Surprising insights have been obtained by comparison of the structures and mechanisms of tRNA endonuclease from these two divergent lines.
|Additional Information:||Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.|
|Subject Keywords:||TRANSFER-RNA PRECURSORS; GROUP-I INTRON; SACCHAROMYCES-CEREVISIAE; SUBSTRATE RECOGNITION; INTERVENING SEQUENCES; YEAST LIGASE; ENDONUCLEASE; MUTANT; GENES; CONFORMATION|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Lindsay Cleary|
|Deposited On:||09 Jun 2006|
|Last Modified:||26 Dec 2012 08:54|
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