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Lineage and fate of each blastomere of the eight-cell sea urchin embryo

Cameron, R. Andrew and Hough-Evans, Barbara R. and Britten, Roy J. and Davidson, Eric H. (1987) Lineage and fate of each blastomere of the eight-cell sea urchin embryo. Genes and Development, 1 (1). pp. 75-84. ISSN 0890-9369. http://resolver.caltech.edu/CaltechAUTHORS:20121023-083116845

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Abstract

A fluoresceinated lineage tracer was injected into individual blastomeres of eight-cell sea urchin (Strongylocentrotus purpuratus) embryos, and the location of the progeny of each blastomere was determined in the fully developed pluteus. Each blastomere gives rise to a unique portion of the advanced embryo. We confirm many of the classical assignments of cell fate along the animal-vegetal axis of the cleavage-stage embryo, and demonstrate that one blastomere of the animal quartet at the eight-cell stage lies nearest the future oral pole and the opposite one nearest the future aboral pole of the embryo. Clones of cells deriving from ectodermal founder cells always remain contiguous, while clones of cells descendant from the vegetal plate (i.e., gut, secondary mesenchyme) do not. The locations of ectodermal clones contributed by specific blastomeres require that the larval plane of bilateral symmetry lie approximately equidistant (i.e., at a 45 degree angle) from each of the first two cleavage planes. These results underscore the conclusion that many of the early spatial patterns of differential gene expression observed at the molecular level are specified in a clonal manner early in embryonic sea urchin development, and are each confined to cell lineages established during cleavage.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1101/gad.1.1.75 DOIUNSPECIFIED
http://genesdev.cshlp.org/content/1/1/75PublisherUNSPECIFIED
Additional Information:© 1987 by Cold Spring Harbor Laboratory. Received December 8, 1986; accepted December 24, 1986. We thank Ronald Hill for technical assistance. Robert L. Gimlich kindly provided fluoresceinated dextran. This work was supported by National Science Foundation grant DCB 8544707 to R.A.C., by National Institutes of Health Grant HD-05753 to E.H.D., and by National Institutes of Health Biomedical Research Support grant RR07003.
Funders:
Funding AgencyGrant Number
NSFDCB 8544707
NIHHD-05753
NIH Biomedical Research Support GrantRR07003
Subject Keywords:Development; cell lineage; dye injection; axis specification
Record Number:CaltechAUTHORS:20121023-083116845
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20121023-083116845
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:35020
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:23 Oct 2012 22:12
Last Modified:03 Sep 2014 04:27

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