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Correct Cell-Type-Specific Expression of a Fusion Gene Injected into Sea Urchin Eggs

Hough-Evans, Barbara R. and Franks, Roberta R. and Cameron, R. Andrew and Britten, Roy J. and Davidson, Eric H. (1987) Correct Cell-Type-Specific Expression of a Fusion Gene Injected into Sea Urchin Eggs. Developmental Biology, 121 (2). pp. 576-579. ISSN 0012-1606. http://resolver.caltech.edu/CaltechAUTHORS:20121023-100217450

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Abstract

A fusion gene construct containing the bacterial chloramphenicol acetyltransferase (CAT) gene under the control of CyIIIa actin gene regulatory sequences was injected into unfertilized eggs of the sea urchin Strongylocentrotus purpuratus, and early pluteus stage embryos that developed from these eggs were fixed and sectioned for analysis by in situ hybridization. A [^3H]RNA antisense probe for CAT mRNA was hybridized to 5-μm embryo sections. Autoradiographic signal denoting the presence of CAT mRNA was detected only over aboral ectoderm cells, in which the CyIIIa gene is normally expressed, and not over any recognizable regions of gut or oral ectoderm included in the same sections.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0012-1606(87)90193-XDOIUNSPECIFIED
http://www.sciencedirect.com/science/article/pii/001216068790193XPublisherUNSPECIFIED
Additional Information:© 1987 Academic Press, Inc. Received September 22, 1986; accepted in revised form December 29, 1986. We thank R. C. Angerer and K. H. Cox for helpful consultations on the techniques of in situ hybridization. This research was supported by NIH Grant HD05753. R.R.F. was supported by an NIH postdoctoral training grant (HD07257), R.A.C. by NSF Grant DCB-8544707.
Funders:
Funding AgencyGrant Number
NIHHD05753
NIH postdoctoral training grantHD07257
NSFDCB-8544707
Record Number:CaltechAUTHORS:20121023-100217450
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20121023-100217450
Official Citation:Barbara R. Hough-Evans, Roberta R. Franks, R.Andrew Cameron, Roy J. Britten, Eric H. Davidson, Correct cell-type-specific expression of a fusion gene injected into sea urchin eggs, Developmental Biology, Volume 121, Issue 2, June 1987, Pages 576-579, ISSN 0012-1606, 10.1016/0012-1606(87)90193-X. (http://www.sciencedirect.com/science/article/pii/001216068790193X)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:35025
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:23 Oct 2012 17:24
Last Modified:23 Oct 2012 17:24

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