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Expression of two actin genes during larval development in the sea urchin Strongylocentrotus purpuratus

Cameron, R. Andrew and Britten, Roy J. and Davidson, Eric H. (1989) Expression of two actin genes during larval development in the sea urchin Strongylocentrotus purpuratus. Molecular Reproduction and Development, 1 (3). pp. 149-155. ISSN 1040-452X . http://resolver.caltech.edu/CaltechAUTHORS:20121023-104612076

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Abstract

We report the first measurements of cell number, total RNA, and transcript accumulations for two actin genes during larval development of the sea urchin Strongylocentrotus purpuratus. At 5 weeks of feeding, when development of laboratory-raised larvae is completed, the cell number has increased about 100-fold with respect to the pluteus-stage embryo to about 150,000 ± 50,000, and the total RNA has increased 46-fold to about 130 ng per larva. The transcripts of the Cylla cytoskeletal actin gene, which is expressed in adult tissues, continue to accumulate throughout larval development. A contrasting pattern of transcript accumulation is observed for Cyllla, a different cytoskeletal actin gene that in the embryo is expressed only in aboral ectoderm. These transcripts increase in number early in larval development, when the larval epidermis is differentiating, and then decline in quantity. It is known that at metamorphosis the larval epidermis is largely histolyzed and that the Cyllla gene is not expressed in the juvenile or adult.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1002/mrd.1080010302DOIUNSPECIFIED
http://onlinelibrary.wiley.com/doi/10.1002/mrd.1080010302/abstractPublisherUNSPECIFIED
Additional Information:© 1989 Alan R. Liss, Inc. Received September 16, 1988; accepted November 30, 1988. We thank Ani Ketabgian for expert technical assistance. This work was supported by NIH grant HD 05753.
Funders:
Funding AgencyGrant Number
NIH HD 05753
Subject Keywords:Gene regulation, Echinoderm
Record Number:CaltechAUTHORS:20121023-104612076
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20121023-104612076
Official Citation:Cameron, R. A., Britten, R. J. and Davidson, E. H. (1989), Expression of two actin genes during larval development in the sea urchin Strongylocentrotus purpuratus. Mol. Reprod. Dev., 1: 149–155. doi: 10.1002/mrd.1080010302
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:35028
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:23 Oct 2012 18:25
Last Modified:23 Oct 2012 18:25

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