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Specific in Vitro Interaction between Recombinant Strongylocentrotus purpuratus Bindin and a Recombinant 45A Fragment of the Putative Bindin Receptor

Cameron, R. Andrew and Walkup, Traci Shipley and Rood, Kirsten and Moore, James G. and Davidson, Eric H. (1996) Specific in Vitro Interaction between Recombinant Strongylocentrotus purpuratus Bindin and a Recombinant 45A Fragment of the Putative Bindin Receptor. Developmental Biology, 180 (1). pp. 348-352. ISSN 0012-1606. http://resolver.caltech.edu/CaltechAUTHORS:20121023-111620345

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Abstract

The interaction between recombinant Strongylocentrotus purpuratus sperm bindin and a recombinant fragment of the putative egg bindin receptor of the same species was measured in vitro. In solution these molecules interact with simple bimolecular kinetics, displaying an equilibrium dissociation constant of about 0.1 μM. Thus, as implied by many observations in vivo and in vitro, bindin and the putative egg receptor display a specific affinity for one another.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1006/dbio.1996.0307DOIUNSPECIFIED
http://www.sciencedirect.com/science/article/pii/S0012160696903073PublisherUNSPECIFIED
Additional Information:© 1996 Academic Press. Received for publication: July 22, 1996; accepted September 18, 1996. We are grateful to R. L. Stears and W. J. Lennarz for providing the 45A S. purpuratus receptor construct and to Dr. Lennarz for useful comments. This research was supported by NSF Grant IBN9117535 to E.H.D.
Funders:
Funding AgencyGrant Number
NSFIBN9117535
Record Number:CaltechAUTHORS:20121023-111620345
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20121023-111620345
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:35032
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:23 Oct 2012 21:31
Last Modified:23 Oct 2012 21:31

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