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Expression Pattern of Brachyury and Not in the Sea Urchin: Comparative Implications for the Origins of Mesoderm in the Basal Deuterostomes

Peterson, Kevin J. and Harada, Yoshito and Cameron, R. Andrew and Davidson, Eric H. (1999) Expression Pattern of Brachyury and Not in the Sea Urchin: Comparative Implications for the Origins of Mesoderm in the Basal Deuterostomes. Developmental Biology, 207 (2). pp. 419-431. ISSN 0012-1606. http://resolver.caltech.edu/CaltechAUTHORS:20121023-134712335

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Abstract

This work concerns the expression of two transcription factors during the development of the sea urchin Strongylocentrotus purpuratus: SpNot, the orthologue of the vertebrate Not gene, and SpBra, the orthologue of the vertebrate Brachyury gene. SpNot transcripts are detected by in situ hybridization in the vegetal plate at the mesenchyme–blastula stage. Later the gene is expressed in the secondary mesenchyme, but expression is no longer detectable after gastrulation. SpNot is upregulated during larval development, in the invaginating vestibule of the adult rudiment. Transcripts are also found in several larva-specific tissues, including the epaulets, blastocoelar cells, and pigment cells. SpBra also displays a discontinuous pattern of expression. Much like SpNot, this gene is expressed during embryogenesis in the embryonic vegetal plate and secondary mesenchyme founder cells, and expression is then extinguished. The gene is upregulated over a week later in the feeding larva, in the vestibule of the adult rudiment. In contrast to SpNot, SpBra is also expressed in the mesoderm of both left and right hydrocoels, and it is not expressed in any larva-specific tissues. We compare the spatial expression profile determined in this study with that of the orthologous Brachyury gene in an indirectly developing enteropneust hemichordate, a representative of the sister group to the echinoderms within the deuterostomes. These observations illuminate the genetic basis underlying the process of maximal indirect development in basal deuterostomes. Finally, Brachyury appears to be an excellent marker for the progeny of the set-aside cells of the sea urchin embryo.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1006/dbio.1998.9177DOIUNSPECIFIED
http://www.sciencedirect.com/science/article/pii/S0012160698991771PublisherUNSPECIFIED
Additional Information:© 1999 Academic Press. Received for publication October 22, 1998; Accepted December 11, 1998. K.J.P. thanks members of the Davidson laboratory, especially Andrew Ransick, for their advice with in situ hybridization. We are grateful to Gayle Zborowski for her expert technical assistance. We thank Professor Gregory Wray (SUNY, Stony Brook) and Dr. Andrew Ransick (Caltech) for review and advice on a draft of the manuscript. This work was supported by grants from the NIH (HD-05753) to E.H.D. and NSF (IBN-9604454) to R.A.C. K.J.P. was supported by a Caltech Molecular Geobiology Fellowship.
Funders:
Funding AgencyGrant Number
NIHHD-05753
NSFIBN-9604454
Caltech Molecular Geobiology FellowshipUNSPECIFIED
Subject Keywords:Brachyury; Not; sea urchin; evolution; mesoderm; indirect development
Record Number:CaltechAUTHORS:20121023-134712335
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20121023-134712335
Official Citation:Kevin J Peterson, Yoshito Harada, R.Andrew Cameron, Eric H Davidson, Expression Pattern of Brachyury and Not in the Sea Urchin: Comparative Implications for the Origins of Mesoderm in the Basal Deuterostomes, Developmental Biology, Volume 207, Issue 2, 15 March 1999, Pages 419-431, ISSN 0012-1606, 10.1006/dbio.1998.9177.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:35040
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:23 Oct 2012 21:17
Last Modified:23 Oct 2012 21:17

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