Summers, Robert G. and Stricker, Stephen A. and Cameron, R. Andrew (1993) Applications of Confocal Microscopy to Studies of Sea Urchin Embryogenesis. In: Cell Biological Applications of Confocal Microscopy. Methods in Cell Biology. No.38. Academic Press , San Diego, pp. 265-287. ISBN 978-0-12-564138-8 http://resolver.caltech.edu/CaltechAUTHORS:20121024-090445621
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The principles, advantages, and some of the general applications of confocal microscopy are presented by Wright et al. ([I] in this volume). The primary benefit of the confocal microscope derives from its ability to obtain optical sections from which out-of-focus information has been removed by the confocal aperture. The background rejection of the confocal aperture is only one consideration, however. Specimen preparation is an equally important factor that determines whether or not sections of sufficient resolution and contrast can be successfully obtained from deep within a thick specimen (see Cheng and Summers, 1990). Once confocal images of adequate quality are collected from the interior of an entire specimen or from a limited area of interest within that sample, the optical sections are stored individually in a digital form on the host computer. Subsequently, these data sets can be transformed into three-dimensional, stereoscopic, or extended-focus views that allow an entire specimen to be viewed in focus (Boyde, 1990).
|Item Type:||Book Section|
|Additional Information:||© 1993 Academic Press, Inc. R.G.S. thanks Alan Stonebraker. David Piston. Michael Marko, Ardean Leith, and John B. Morrill for collaborative assistance. Technical assistance was provided by Thaddeus Szczesny and Edward Hurley. Project supported by NIH RR 06993 to R.G.S. and by NIH Biotechnological Resource Grants RR01219 to the NIH Biological Microscopy and Image Reconstruction Resource (Albany, NY) and RR04224 to the Developmental Resource for Biophysical Imaging and Optoelectronics at Cornell University (Ithaca, NY). S.A.S. gratefully acknowledges V. Centonze, P. DeVries, S. Paddock, and G. Schatten. R.A.C. wishes to thank E. H. Davidson and S. E. Fraser for their collaboration. The original research discussed here was supported by the NSF Program in Developmental Biology.|
|Official Citation:||Robert G. Summers, Stephen A. Stricker, R. Andrew Cameron, Chapter 10 Applications of Confocal Microscopy to Studies of Sea Urchin Embryogenesis, In: Brian Matsumoto, Editor(s), Methods in Cell Biology, Academic Press, 1993, Volume 38, Pages 265-287, ISSN 0091-679X, ISBN 9780125641388, 10.1016/S0091-679X(08)61007-9. (http://www.sciencedirect.com/science/article/pii/S0091679X08610079)|
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|Deposited By:||Ruth Sustaita|
|Deposited On:||24 Oct 2012 16:36|
|Last Modified:||19 Oct 2015 19:30|
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