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Microsatellite Loci in Wild-Type and Inbred Strongylocentrotus purpuratus

Cameron, R. Andrew and Leahy, Patrick S. and Britten, Roy J. and Davidson, Eric H. (1999) Microsatellite Loci in Wild-Type and Inbred Strongylocentrotus purpuratus. Developmental Biology, 208 (2). pp. 255-264. ISSN 0012-1606. http://resolver.caltech.edu/CaltechAUTHORS:20121025-093551154

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Abstract

Strongylocentrotus purpuratus,a major research model in developmental molecular biology, has been inbred through six generations of sibling matings. Though viability initially decreased, as described earlier, the inbred line now consists of healthy, fertile animals. These are intended to serve as a genomic resource in which the level of polymorphism is decreased with respect to wildS. purpuratus.To genotype the inbred animals eight simple sequence genomic repeats were isolated, in context, and PCR primers were generated against the flanking single-copy sequences. Distribution and polymorphism of these regions of the genome were studied in the genomes of 27 wild individuals and in a sample of the inbred animals at F2 and F3 generations. All eight regions were polymorphic, though to different extents, and their homozygosity was increased by inbreeding as expected. The eight markers suffice to identify unambiguously the cellular DNA of any wild or F3S. purpuratus individual.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1006/dbio.1999.9224DOIUNSPECIFIED
http://www.sciencedirect.com/science/article/pii/S0012160699992242PublisherUNSPECIFIED
Additional Information:© 1999 Academic Press. Received for publication December 3, 1998. Revised January 27, 1999. Accepted January 27, 1999. Traci Walkup and Jane Wyllie provided expert technical assistance. We thank Drs. Richard Grosberg and Robert Toonen for their comments on the manuscript. This work was supported by grants from the NIH (RR06591) and the California Sea Grant Program (NA66RG0477).
Funders:
Funding AgencyGrant Number
NIHRR06591
California Sea Grant ProgramNA66RG0477
Record Number:CaltechAUTHORS:20121025-093551154
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20121025-093551154
Official Citation:R.Andrew Cameron, Patrick S. Leahy, Roy J. Britten, Eric H. Davidson, Microsatellite Loci in Wild-Type and InbredStrongylocentrotus purpuratus, Developmental Biology, Volume 208, Issue 2, 15 April 1999, Pages 255-264, ISSN 0012-1606, 10.1006/dbio.1999.9224. (http://www.sciencedirect.com/science/article/pii/S0012160699992242)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:35087
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:25 Oct 2012 16:48
Last Modified:25 Oct 2012 16:48

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