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Cloning and analysis of reverse transcript P160 genomes of Abelson murine leukemia virus

Latt, Samuel A. and Goff, Stephen P. and Tabin, Clifford J. and Paskind, Michael and Wang, Jean Y.-J. and Baltimore, David (1983) Cloning and analysis of reverse transcript P160 genomes of Abelson murine leukemia virus. Journal of Virology, 45 (3). pp. 1195-1199. ISSN 0022-538X. http://resolver.caltech.edu/CaltechAUTHORS:LATjvir83

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Abstract

Circular duplex reverse transcripts of the genome of a strain of Abelson murine leukemia virus that encodes a 160,000-molecular-weight protein were isolated, cleaved with HindIII restriction endonuclease, and cloned into the unique HindIII site of lambda phage Charon 21A. Recombinant phage clones, some of which were infectious in transfection assays, were found to contain a 789-base-pair region specific for Abelson murine leukemia virus; this region is not found in other strains of this virus. The extra sequence was localized by restriction endonuclease and electron microscopic heteroduplex analysis. Sequence analysis showed no homology at the ends of the extra sequence, implying that it was deleted by an event that did not utilize sequence homology. The sequence of this unique region has an open reading frame through its entirety.


Item Type:Article
Additional Information:Copyright © 1983 by the American Society for Microbiology. Received 27 September 1982. Accepted 22 November 1982. We thank Chris Riser for advice on the use of the electron microscope. This work was supported by Public Health Service grants CA-26717 (to D.B.), GM-21121 (to S.A.L.), and CA-14501 (core grant to S. E. Luria) from the National Institutes of Health. S.P.G. was a postdoctoral fellow of the Jane Coffin Childs Memorial Fund for Medical Research, as is J.Y.-J.W. D.B. is an American Cancer Society Research Professor.
Record Number:CaltechAUTHORS:LATjvir83
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:LATjvir83
Alternative URL:http://jvi.asm.org/cgi/content/abstract/45/3/1195
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:3620
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:21 Jun 2006
Last Modified:26 Dec 2012 08:55

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