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Isolation and properties of Moloney murine leukemia virus mutants: use of a rapid assay for release of virion reverse transcriptase

Goff, Stephen and Traktman, Paula and Baltimore, David (1981) Isolation and properties of Moloney murine leukemia virus mutants: use of a rapid assay for release of virion reverse transcriptase. Journal of Virology, 38 (1). pp. 239-248. ISSN 0022-538X. http://resolver.caltech.edu/CaltechAUTHORS:GOFjvir81a

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Abstract

A rapid assay for retroviral reverse transcriptase activity released into the culture medium by infected cells was developed. With the assay, 4,000 clonally infected cell lines could be tested in a few hours. We have adapted the assay for use as a screen for the detection of spontaneous viral mutants. Mutants of Moloney murine leukemia virus have been isolated which (i) produce a thermolabile reverse transcriptase, (ii) are temperature sensitive for release of enzyme activity, or (iii) can only productively infect cells already producing gag-related polypeptides. The assay has also been useful for the isolation of nonproducer cells infected with various replication-defective transforming viruses.


Item Type:Article
Additional Information:Copyright © 1981 by the American Society for Microbiology. Received 25 November 1980. Accepted 7 January 1981. We thank Owen Witte for helpful discussions. This work was supported by contract N01-CP-53562 from the Division of Cancer Cause and Prevention, National Cancer Institute, and by Public Health Service grant CA-14051 (core grant to S.E. Luria) from the National Cancer Institute. S.P.G. is a Jane Coffin Childs Memorial Fund Fellow. D.B. is an American Cancer Society Research Professor.
Record Number:CaltechAUTHORS:GOFjvir81a
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:GOFjvir81a
Alternative URL:http://jvi.asm.org/cgi/content/abstract/38/1/239
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:3623
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:22 Jun 2006
Last Modified:26 Dec 2012 08:55

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