CaltechAUTHORS
  A Caltech Library Service

Active sulfur cycling by diverse mesophilic and thermophilic microorganisms in terrestrial mud volcanoes of Azerbaijan

Green-Saxena, A. and Feyzullayev, A. and Hubert, C. R. J. and Kallmeyer, J. and Krueger, M. and Sauer, P. U. and Schulz, H.-M. and Orphan, V. J. (2012) Active sulfur cycling by diverse mesophilic and thermophilic microorganisms in terrestrial mud volcanoes of Azerbaijan. Environmental Microbiology, 14 (12). pp. 3271-3286. ISSN 1462-2912. http://resolver.caltech.edu/CaltechAUTHORS:20130108-135620155

[img]
Preview
Image (TIFF) (Figure S1) - Supplemental Material
See Usage Policy.

1495Kb
[img]
Preview
Image (TIFF) (Figure S2) - Supplemental Material
See Usage Policy.

134Kb
[img] MS Word (Table S1) - Supplemental Material
See Usage Policy.

30Kb
[img] MS Word (Table S2) - Supplemental Material
See Usage Policy.

312Kb
[img] MS Word (Table S3) - Supplemental Material
See Usage Policy.

33Kb
[img] MS Word (Table S4) - Supplemental Material
See Usage Policy.

72Kb
[img] MS Word (Table S5) - Supplemental Material
See Usage Policy.

60Kb

Use this Persistent URL to link to this item: http://resolver.caltech.edu/CaltechAUTHORS:20130108-135620155

Abstract

Terrestrial mud volcanoes (TMVs) represent geochemically diverse habitats with varying sulfur sources and yet sulfur cycling in these environments remains largely unexplored. Here we characterized the sulfur-metabolizing microorganisms and activity in four TMVs in Azerbaijan. A combination of geochemical analyses, biological rate measurements and molecular diversity surveys (targeting metabolic genes aprA and dsrA and SSU ribosomal RNA) supported the presence of active sulfur-oxidizing and sulfate-reducing guilds in all four TMVs across a range of physiochemical conditions, with diversity of these guilds being unique to each TMV. The TMVs varied in potential sulfate reduction rates (SRR) by up to four orders of magnitude with highest SRR observed in sediments where in situ sulfate concentrations were highest. Maximum temperatures at which SRR were measured was 60°C in two TMVs. Corresponding with these trends in SRR, members of the potentially thermophilic, spore-forming, Desulfotomaculum were detected in these TMVs by targeted 16S rRNA analysis. Additional sulfate-reducing bacterial lineages included members of the Desulfobacteraceae and Desulfobulbaceae detected by aprA and dsrA analyses and likely contributing to the mesophilic SRR measured. Phylotypes affiliated with sulfide-oxidizing Gamma- and Betaproteobacteria were abundant in aprA libraries from low sulfate TMVs, while the highest sulfate TMV harboured 16S rRNA phylotypes associated with sulfur-oxidizing Epsilonproteobacteria. Altogether, the biogeochemical and microbiological data indicate these unique terrestrial habitats support diverse active sulfur-cycling microorganisms reflecting the in situ geochemical environment.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1111/1462-2920.12015 DOIArticle
http://onlinelibrary.wiley.com/doi/10.1111/1462-2920.12015/abstractPublisherArticle
ORCID:
AuthorORCID
Orphan, V. J.0000-0002-5374-6178
Additional Information:© 2012 Society for Applied Microbiology and Blackwell Publishing Ltd. Received 5 March, 2012; revised 9 July, 2012; accepted 28 September, 2012. Article first published online: 1 Nov. 2012. We would like to acknowledge Dr Chingiz Aliyev and Rauf Bagirli for assistance during field trip and laboratory investigations in Baku; Daniela Zoch and Holger Probst for technical work at BGR, and Andrea Vieth-Hillebrandt and Kristin Günther for water analysis at Helmholtz Centre Potsdam GFZ. Funding for this work was provided by a DOE Career grant (to V.J.O.), a NSF GRFP (to A.G.-S.) and through the Forschungsverbund GeoEnergie of the German Ministry for Education and Research (BMBF, to J.K. and P.S.). We would also like to thank two anonymous reviewers for their valuable input.
Funders:
Funding AgencyGrant Number
Department of Energy (DOE)UNSPECIFIED
NSF Graduate Research FellowshipUNSPECIFIED
Bundesministerium für Bildung und Forschung (BMBF)UNSPECIFIED
Record Number:CaltechAUTHORS:20130108-135620155
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20130108-135620155
Official Citation:Green-Saxena, A., Feyzullayev, A., Hubert, C. R. J., Kallmeyer, J., Krueger, M., Sauer, P., Schulz, H.-M. and Orphan, V. J. (2012), Active sulfur cycling by diverse mesophilic and thermophilic microorganisms in terrestrial mud volcanoes of Azerbaijan. Environmental Microbiology, 14: 3271–3286. doi: 10.1111/1462-2920.12015
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:36242
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:09 Jan 2013 19:21
Last Modified:09 Jan 2016 03:29

Repository Staff Only: item control page