Minor, Joseph E. and Britten, Roy J. and Davidson, Eric H. (1993) Species-specific inhibition of fertilization by a peptide derived from the sperm protein bindin. Molecular Biology of the Cell, 4 (4). pp. 375-387. ISSN 1059-1524 http://resolver.caltech.edu/CaltechAUTHORS:MINmbc93
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The sperm protein bindin is responsible for the species-specific adhesion of the sperm to the egg. The regions of the bindin molecule responsible for forming the contact between the sperm and the egg were investigated by measuring the ability of peptides representing various regions of the bindin sequence to inhibit fertilization. Twenty-four peptides were studied: 7 based on the Strongylocentrotus purpuratus bindin sequence, 11 based on the S. franciscanus bindin sequence, and 6 control peptides. Values for the concentration of peptide required to inhibit 50% of the productive sperm contacts (IC50) were extracted from experimental measurements of the extent of fertilization in the presence of various concentrations. of these peptides. The IC50 value averaged 220 microM for the control peptides. Active peptides representing certain specific subregions of the bindin sequence displayed IC50 values < 10% of the average value for control peptides, and the IC50 for the most potent of the peptides tested was only approximately 1% of the control peptide value (IC50 = 2.2 microM). Furthermore, we found that a peptide representing a particular region of the S. franciscanus bindin sequence that differs from the S. purpuratus bindin sequence inhibits fertilization species specifically. For the reaction of S. purpuratus sperm and eggs, the IC50 of this peptide was approximately 120 microM, whereas for the reaction of S. franciscanus sperm and eggs it was only 8.6 microM. These results demonstrate that a few specific regions of the bindin molecule are involved in the sperm-egg contact and that certain of these regions mediate the species specificity of the interaction in a sequence- specific manner.
|Additional Information:||Copyright © 1993 by The American Society for Cell Biology Submitted January 6, 1993; Accepted February 9, 1993 We thank Dr. Peter von Hippel for advising us both on the method of data analysis and on its interpretation. We also thank Dr. Lou DeFelice for helpful comments on the manuscript, and James G. Moore for help with data reduction. Control peptides CP5 and CP6 were a gift from Li-Ching Lo and Dr. David Anderson. This research was supported by NSF grant DCB-8813413.|
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|Deposited On:||18 Jul 2006|
|Last Modified:||26 Dec 2012 08:56|
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