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Homologous and unique G protein alpha subunits in the nematode Caenorhabditis elegans

Lochrie, Michael A. and Mendel, Jane E. and Sternberg, Paul W. and Simon, Melvin I. (1991) Homologous and unique G protein alpha subunits in the nematode Caenorhabditis elegans. Cell Regulation, 2 (2). pp. 135-154. ISSN 1044-2030. http://resolver.caltech.edu/CaltechAUTHORS:LOCcr91

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Abstract

A cDNA corresponding to a known G protein alpha subunit, the alpha subunit of Go (Go alpha), was isolated and sequenced. The predicted amino acid sequence of C. elegans Go alpha is 80-87% identical to other Go alpha sequences. An mRNA that hybridizes to the C. elegans Go alpha cDNA can be detected on Northern blots. A C. elegans protein that crossreacts with antibovine Go alpha antibody can be detected on immunoblots. A cosmid clone containing the C. elegans Go alpha gene (goa-1) was isolated and mapped to chromosome I. The genomic fragments of three other C. elegans G protein alpha subunit genes (gpa-1, gpa-2, and gpa-3) have been isolated using the polymerase chain reaction. The corresponding cosmid clones were isolated and mapped to disperse locations on chromosome V. The sequences of two of the genes, gpa-1 and gpa-3, were determined. The predicted amino acid sequences of gpa-1 and gpa-3 are only 48% identical to each other. Therefore, they are likely to have distinct functions. In addition they are not homologous enough to G protein alpha subunits in other organisms to be classified. Thus C. elegans has G proteins that are identifiable homologues of mammalian G proteins as well as G proteins that appear to be unique to C. elegans. Study of identifiable G proteins in C. elegans may result in a further understanding of their function in other organisms, whereas study of the novel G proteins may provide an understanding of unique aspects of nematode physiology.


Item Type:Article
Additional Information:Copyright © 1991 by The American Society for Cell Biology Received: October 5, 1990. Revised and accepted: November 16, 1990. We thank Mike Strathmann for advice on PCR reactions and for the provision of materials for the γδ transposon-based sequencing method, the Caltech Microchemical Facility for oligonucleotide synthesis, Andrea Holboke for C. elegans genomic DNA, Alan Coulson and John Sulston for gene mapping data, Eva Neer and Graeme Milligan for G0α antibodies, Stuart Kim and Russell Hill for the C. elegans cDNA library, and Yasumi Ohshima and Ron Plasterk for generous communication of results before publication. P.W.S. is an investigator of the Howard Hughes Medical Institute. Supported by a Developmental Biology Grant from the Lucille P. Markey Foundation and by USPHS grants GM34236 to M.I.S. and HD23690 to P.W.S.
Record Number:CaltechAUTHORS:LOCcr91
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:LOCcr91
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ID Code:3903
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Deposited On:18 Jul 2006
Last Modified:26 Dec 2012 08:56

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