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Fractionation of human immune γ-globulin

Cann, John R. and Brown, Raymond A. and Kirkwood, John G. and Hink, John H., Jr. (1950) Fractionation of human immune γ-globulin. Journal of Biological Chemistry, 185 (2). pp. 663-667. ISSN 0021-9258. http://resolver.caltech.edu/CaltechAUTHORS:CANjbc50

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Abstract

Equine and bovine serum proteins have recently been fractionated by means of a physical method utilizing an electrophoretic adaptation of the principles of the Clusius column (l-4), first described and tested by Kirkwood (5) and Nielsen (6). The method of electrophoresis-convection has now been applied to the fractionation of human γ-globulin. The γ-globulin was prepared by ethanol fractionation (7) from the plasma of individuals hyperimmunized to Hemophilus pertussis organisms. The resulting fractions of γ-globulin have been characterized electrophoretically, and the protective antibody activity and agglutinin titer have been measured.


Item Type:Article
Additional Information:Copyright © 1950 by the American Society of Biological Chemists. (Received for publication, February 18, 1950) The authors are indebted to Dr. Dan Campbell for helpful discussion of the immunological significance of the results.
Record Number:CaltechAUTHORS:CANjbc50
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:CANjbc50
Alternative URL:http://www.jbc.org/content/vol185/issue2/index.shtml
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:3996
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:24 Jul 2006
Last Modified:26 Dec 2012 08:57

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