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Adenine specific DNA chemical sequencing reaction

Iverson, Brent L. and Dervan, Peter B. (1987) Adenine specific DNA chemical sequencing reaction. Nucleic Acids Research, 15 (19). pp. 7823-7830. ISSN 0305-1048. http://resolver.caltech.edu/CaltechAUTHORS:IVEnar87

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Abstract

Reaction of DNA with K2PdCI4 at pH2.0 followed by a piperidine workup produces specific cleavage at adenine (A) residues. Product analysis revealed the K2PdCI4 reaction involves selective depurination at adenine, affording an excision reaction analogous to the other chemical DNA sequencing reactions. Adenine residues methylated at the exocyclic amine (N6) react with lower efficiency than unmethylated adenine in an identical sequence. This simple protocol specific for A may be a useful addition to current chemical sequencing reactions.


Item Type:Article
Additional Information:Copyright © 1997 Oxford University Press. Received July 13, 1987. Accepted August 30, 1987. We are grateful to the American Cancer Society (NP 428) for support of this research and for a National Research Service Award to B.L.I. from the National Institute of General Medical Sciences.
Record Number:CaltechAUTHORS:IVEnar87
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:IVEnar87
Alternative URL:http://nar.oxfordjournals.org/cgi/content/abstract/15/19/7823
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:4010
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:24 Jul 2006
Last Modified:26 Dec 2012 08:57

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