A Caltech Library Service

A program for least squares analysis of reassociation and hybridization data

Pearson, W. R. and Davidson, E. H. and Britten, R. J. (1977) A program for least squares analysis of reassociation and hybridization data. Nucleic Acids Research, 4 (6). pp. 1727-1737. ISSN 0305-1048.

See Usage Policy.


Use this Persistent URL to link to this item:


A computer program is described for the rapid calculation of least squares solutions for data fitted to different functions normally used in reassociation and hybridization kinetic measurements. The equations for the fraction not reacted as a function of Cot follow: First order, exp(–kCot); second order, (1+kCot)–1 ; variable order, (1+kCot)–n; approximate fraction of DNA sequence remaining single stranded, (1+kCot)–44; and a function describing the pairing of tracer when the rate constant for the tracer (k) is distinct from the driver rate constant kd): exp{kL1-(1+kdCot)1–nJ/Lkd(1-n)J}. Several components may be used for most of these functional forms. The standard deviations of the individual parameters at the solutions are calculated.

Item Type:Article
Additional Information:Copyright © 1977 Oxford University Press. Received December 21, 1976.
Record Number:CaltechAUTHORS:PEAnar77
Persistent URL:
Alternative URL:
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:4037
Deposited By: Tony Diaz
Deposited On:25 Jul 2006
Last Modified:26 Dec 2012 08:57

Repository Staff Only: item control page