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Hybridization of synthetic oligodeoxyribonucleotides to ΦX 174 DNA: the effect of single base pair mismatch

Wallace, R. Bruce and Shaffer, J. and Murphy, R. F. and Bonner, J. and Hirose, T. and Itakura, K. (1979) Hybridization of synthetic oligodeoxyribonucleotides to ΦX 174 DNA: the effect of single base pair mismatch. Nucleic Acids Research, 6 (11). pp. 3543-3557. ISSN 0305-1048. http://resolver.caltech.edu/CaltechAUTHORS:WALnar79

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Abstract

Oligodeoxyribonucleotides complementary to the DNA of the wild type (wt) bacteriophage ΦX174 have been synthesized by the phosphotriester method. The ollgomers, 11, 14, and 17 bases long, are complementary to the region of the DNA which accounts for the am-3 point mutation. When hybridized to am-3 DNA, the oligonucleotides form duplexes with a single base pair mismatch. The thermal stability of the duplexes formed between wt and am-3 DNAs has been measured. The am-3 DNA: ollgomer duplexes dissociate at a temperature about 10°C lower than the corresponding wt DNA: ollgomer duplexes. This dramatic decrease in thermal stability due to a single mismatch makes it possible to eliminate the formation of the mismatched duplexes by the appropriate choice of hybridization temperature. These results are discussed with respect to the use of oligonucleotides as probes for the isolation of specific cloned DNA sequences.


Item Type:Article
Additional Information:Copyright © 1979 Oxford University Press. Received April 16, 1979. This work was supported by USPHS grants to James Bonner (GM-13762) and Keiichi Itakura (GM-25658) and funds from NIH Biomedical Research Support Grant 5S07 RR05471-16 to the City of Hope National Medical Center. We are grateful to the excellent technical assistance of Merrie Jo Johnson.
Record Number:CaltechAUTHORS:WALnar79
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:WALnar79
Alternative URL:http://nar.oxfordjournals.org/cgi/content/abstract/6/11/3543
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:4058
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:26 Jul 2006
Last Modified:26 Dec 2012 08:57

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