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Alignment of the amino terminal amino acid sequence of human cytochrome c oxidase subunits I and II with the sequence of their putative mRNAs

Chomyn, Anne and Hunkapiller, Michael W. and Attardi, Giuseppe (1981) Alignment of the amino terminal amino acid sequence of human cytochrome c oxidase subunits I and II with the sequence of their putative mRNAs. Nucleic Acids Research, 9 (4). pp. 867-877. ISSN 0305-1048. http://resolver.caltech.edu/CaltechAUTHORS:CHOnar81

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Abstract

Thirteen of the first fifteen amino acids from the NH2-terminus of the primary sequence of human cytochrome c oxidase subunit I and eleven of the first twelve amino acids of subunit II have been identified by microsequencing procedures. These sequences have been compared with the recently determined 5'-end proximal sequences of the HeLa cell mitochondrial mRNAS and unambiguously aligned with two of them. This alignment has allowed the identification of the putative mRNA for subunit I, and has shown that the initiator codon for this subunit is only three nucleotides away from the 5'-end of its mRNA; furthermore, the results have substantiated the idea that the translation of human cytochrome c oxidase subunit II starts directly at the 5'-end of its putative mRNA, as had been previously inferred on the basis of the sequence homology of human mitochondrial DNA with the primary sequence of the bovine subunit.


Item Type:Article
Additional Information:Copyright © 1981 Oxford University Press. Received 1 December 1980. This work was supported by research grants from the USPHS (GM-11726) and the Weingart Foundation and was done under the tenure of a Research Fellowship of the American Heart Association, Greater Los Angeles Affiliate, to A. C. We are very grateful to Drs. F. Sanger and B. Barrell for communicating to us mitochondrial DNA sequence data and sending us a manuscript prior to publication and to Dr. E. Ching for a gift of bovine cytochrome c oxidase.
Record Number:CaltechAUTHORS:CHOnar81
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:CHOnar81
Alternative URL:http://nar.oxfordjournals.org/cgi/content/abstract/9/4/867
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:4065
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:26 Jul 2006
Last Modified:26 Dec 2012 08:57

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