Seed, Brian (1982) Diazotizable arylamine cellulose papers for the coupling and hybridization of nucleic acids. Nucleic Acids Research, 10 (5). pp. 1799-1810. ISSN 0305-1048 http://resolver.caltech.edu/CaltechAUTHORS:SEEnar82
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Abstract
We describe the synthesis of a family of arylamine-substituted papers which can be diazotized and coupled with nucleic acids. The synthesis is simple and uses readily obtainable starting materials. A partial characterization of the nucleic acid binding activity is reported, as well as a demonstration of the utility of the activated paper for the detection of electrophoretically separated RNA by blot transfer and hybridization.
| Item Type: | Article |
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| Additional Information: | Copyright © 1982 Oxford University Press. Received November 10, 1981. Revised February 5, 1982. Accepted February 5, 1982. I would like to thank the many people who shared their experiences with me, traumatic and otherwise; and among them particularly Jim Casey, Chien Yueh-Hsieh, David Goldberg, Ross Hardison, and Liz Lacy. I would also like to thank Tom Maniatis for laboratory space, and my parents for support. |
| Record Number: | CaltechAUTHORS:SEEnar82 |
| Persistent URL: | http://resolver.caltech.edu/CaltechAUTHORS:SEEnar82 |
| Alternative URL: | http://nar.oxfordjournals.org/cgi/content/abstract/10/5/1799 |
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. |
| ID Code: | 4079 |
| Collection: | CaltechAUTHORS |
| Deposited By: | Tony Diaz |
| Deposited On: | 26 Jul 2006 |
| Last Modified: | 26 Dec 2012 08:57 |
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