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Diazotizable arylamine cellulose papers for the coupling and hybridization of nucleic acids

Seed, Brian (1982) Diazotizable arylamine cellulose papers for the coupling and hybridization of nucleic acids. Nucleic Acids Research, 10 (5). pp. 1799-1810. ISSN 0305-1048. http://resolver.caltech.edu/CaltechAUTHORS:SEEnar82

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Abstract

We describe the synthesis of a family of arylamine-substituted papers which can be diazotized and coupled with nucleic acids. The synthesis is simple and uses readily obtainable starting materials. A partial characterization of the nucleic acid binding activity is reported, as well as a demonstration of the utility of the activated paper for the detection of electrophoretically separated RNA by blot transfer and hybridization.


Item Type:Article
Additional Information:Copyright © 1982 Oxford University Press. Received November 10, 1981. Revised February 5, 1982. Accepted February 5, 1982. I would like to thank the many people who shared their experiences with me, traumatic and otherwise; and among them particularly Jim Casey, Chien Yueh-Hsieh, David Goldberg, Ross Hardison, and Liz Lacy. I would also like to thank Tom Maniatis for laboratory space, and my parents for support.
Record Number:CaltechAUTHORS:SEEnar82
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:SEEnar82
Alternative URL:http://nar.oxfordjournals.org/cgi/content/abstract/10/5/1799
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:4079
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:26 Jul 2006
Last Modified:26 Dec 2012 08:57

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