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Purification of genomic sequences from bacteriophage libraries by recombination and selection in vivo

Seed, Brian (1983) Purification of genomic sequences from bacteriophage libraries by recombination and selection in vivo. Nucleic Acids Research, 11 (8). pp. 2427-2445. ISSN 0305-1048. http://resolver.caltech.edu/CaltechAUTHORS:SEEnar83

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Abstract

Cloned genes have been purified from recombinant DNA bacteriophage libraries by a method exploiting homologous reciprocal recombination in vivo. In this method 'probe' sequences are inserted in a very small plasmid vector and introduced into recombination-proficient bacterial cells. Genomic bacteriophage libraries are propagated on the cells, and phage bearing sequences homologous to the probe acquire an integrated copy of the plasmid by reciprocal recombination. Phage bearing integrated plasmids can be purified from the larger pool of phage lacking plasmid integrates by growth under the appropriate selective conditions.


Item Type:Article
Additional Information:Copyright © 1983 Oxford University Press. Received 14 December 1982; Revised and Accepted 24 February 1983. I would like to thank Dan DiMaio, David Goldberg, Henry Huang, Peter Little and Tom Maniatis for suggestions, strains and support. I would particularly like to thank Mark Silver for his assistance in the construction of these plasmids. This work was carried out in Tom Maniatis' laboratory, and was supported in part by NIH grants HL24380 and HL27898 to T.M.
Record Number:CaltechAUTHORS:SEEnar83
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:SEEnar83
Alternative URL:http://nar.oxfordjournals.org/content/vol11/issue8/index.dtl
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:4080
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:26 Jul 2006
Last Modified:26 Dec 2012 08:57

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