Morrison, Trudy and Stampfer, Martha and Baltimore, David and Lodish, Harvey F. (1974) Translation of vesicular stomatitis messenger RNA by extracts from mammalian and plant cells. Journal of Virology, 13 (1). pp. 62-72. ISSN 0022-538X http://resolver.caltech.edu/CaltechAUTHORS:MORjvir74
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RNA was isolated from polyribosomes of vesicular stomatitis virus (VSV)-infected cells and tested for its ability to direct protein synthesis in extracts of animal and plant cells. In cell-free, non-preincubated extracts of rabbit reticulocytes, the 28S VSV RNA stimulated synthesis of a protein the size of the vesicular stomatitis virus L protein whereas the 13 to 15S RNA directed synthesis of the VSV M, N, NS, and possibly G proteins. In wheat germ extracts, 13 to 15S RNA also directed synthesis of the N, NS, M, and possibly G proteins. Analysis of extracts labeled with formyl [35S]methionine showed that the 28S RNA directed the initiation of synthesis of one protein, whereas the 13 to 15S RNA directed initiation of at least four proteins. It is concluded that the 28S RNA encodes only the L protein, whereas the 13 to 15S RNA is a mixture of species, presumably monocistronic, which code for the four other known vesicular stomatitis virus proteins.
|Additional Information:||Copyright © 1974 American Society for Microbiology. Received for publication 20 August 1973. We thank Tom Alton for the preparation of wheat germ extracts. This work was supported by American Cancer Society grant no. VC-4D and Public Health Service grant AI-08814 from the National Institute of Allergy and Infectious Diseases. T. M. is a postdoctoral fellow of the Damon Runyan Memorial Foundation; D. B. is an American Cancer Society Research Professor; H. F. L. is a recipient of Public Health Service research career development award GM-50175 from the National Institute of General Medical Sciences.|
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|Deposited By:||Lindsay Cleary|
|Deposited On:||08 Aug 2006|
|Last Modified:||26 Dec 2012 08:58|
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