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Tachykinin-Expressing Neurons Control Male-Specific Aggressive Arousal in Drosophila

Asahina, Kenta and Watanabe, Kiichi and Duistermars, Brian J. and Hoopfer, Eric and González, Carlos Roberto and Eyjólfsdóttir, Eyrún Arna and Perona, Pietro and Anderson, David J. (2014) Tachykinin-Expressing Neurons Control Male-Specific Aggressive Arousal in Drosophila. Cell, 156 (1-2). pp. 221-235. ISSN 0092-8674. http://resolver.caltech.edu/CaltechAUTHORS:20140117-074512467

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Abstract

Males of most species are more aggressive than females, but the neural mechanisms underlying this dimorphism are not clear. Here, we identify a neuron and a gene that control the higher level of aggression characteristic of Drosophila melanogaster males. Males, but not females, contain a small cluster of FruM+ neurons that express the neuropeptide tachykinin (Tk). Activation and silencing of these neurons increased and decreased, respectively, intermale aggression without affecting male-female courtship behavior. Mutations in both Tk and a candidate receptor, Takr86C, suppressed the effect of neuronal activation, whereas overexpression of Tk potentiated it. Tk neuron activation overcame reduced aggressiveness caused by eliminating a variety of sensory or contextual cues, suggesting that it promotes aggressive arousal or motivation. Tachykinin/Substance P has been implicated in aggression in mammals, including humans. Thus, the higher aggressiveness of Drosophila males reflects the sexually dimorphic expression of a neuropeptide that controls agonistic behaviors across phylogeny.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.cell.2013.11.045DOIArticle
http://www.sciencedirect.com/science/article/pii/S0092867413015365PublisherArticle
Additional Information:© 2014 Elsevier Inc. Received: June 25, 2013; Revised: September 10, 2013; Accepted: November 15, 2013; Published: January 16, 2014. We thank B.J. Dickson, B. Pfeiffer, G.M. Rubin, and D. Nässel for sharing fly strains and antibodies; S. Jeeda and C. Khanbijian for maintenance of fly stocks; G. Mancuso for administrative support; C. Chiu for laboratory management; and B. Pfeiffer, A.M. Wong, and W. Hong for helpful comments on the manuscript. K.A. was a JSPS Postdoctoral Fellow for Research Abroad. K.W. was a Human Frontier Science Program Postdoctoral Fellow. B.J.D. is an Ellison Medical Foundation Fellow of the Life Science Research Foundation. E.H. is supported by an NRSA postdoctoral fellowship. This research was supported in part by NIH grant R01-DA031389 to D.J.A. and a Moore Foundation grant to D.J.A. and P.P. D.J.A. is a Howard Hughes Medical Institute investigator. Author Contributions: K.A. generated most new transgenic animals and mutants, performed all experiments, and cowrote the manuscript. K.W. generated the Otd-nls:FLPo transgenic strain. B.J.D. set up the ‘‘moving magnet’’ arena and performed experiments for Figure 7H with K.A. E.H. established the 12-well aggression chamber assay system, including modification of CADABRA software to run on computer clusters. E.A.E. created a new fly tracking program. C.R.G., with feedback from K.A., wrote a code for the unilateral wing extension classifier. P.P. supervised E.A.E. and C.R.G. and provided useful suggestions to K.A., E.H., and D.J.A. D.J.A. supervised the project and cowrote the manuscript.
Funders:
Funding AgencyGrant Number
JSPS Postdoctoral Fellowship for Research AbroadUNSPECIFIED
Human Frontier Science Program Postdoctoral FellowshipUNSPECIFIED
Ellison Medical Foundation FellowshipUNSPECIFIED
Life Science Research FoundationUNSPECIFIED
NRSA Postdoctoral FellowshipUNSPECIFIED
NIHR01-DA031389
Gordon and Betty Moore FoundationUNSPECIFIED
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Record Number:CaltechAUTHORS:20140117-074512467
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20140117-074512467
Official Citation:Kenta Asahina, Kiichi Watanabe, Brian J. Duistermars, Eric Hoopfer, Carlos Roberto González, Eyrún Arna Eyjólfsdóttir, Pietro Perona, David J. Anderson, Tachykinin-Expressing Neurons Control Male-Specific Aggressive Arousal in Drosophila, Cell, Volume 156, Issues 1–2, 16 January 2014, Pages 221-235, ISSN 0092-8674, http://dx.doi.org/10.1016/j.cell.2013.11.045. (http://www.sciencedirect.com/science/article/pii/S0092867413015365)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:43418
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:17 Jan 2014 17:12
Last Modified:17 Jan 2014 17:12

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