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Studies on the denaturation of antibody: II. The effect of protein concentration on the rate of denaturation of diphtheria antitoxin by urea

Wright, George G. (1945) Studies on the denaturation of antibody: II. The effect of protein concentration on the rate of denaturation of diphtheria antitoxin by urea. Journal of Experimental Medicine, 81 (6). pp. 647-653. ISSN 0022-1007. http://resolver.caltech.edu/CaltechAUTHORS:WRIjem45

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Abstract

The specific rate of inactivation of antitoxin in urea solutions, as measured by the Römer neutralization test with toxin, has been shown to be independent of the concentration of protein under the conditions studied. The amount of precipitate obtained in the quantitative precipitation test with toxin, however, increases greatly with increasing protein concentration during denaturation. The time during which the protein concentration is important in this respect has been shown to be the interval in which the urea is being dialyzed from the solutions. The meaning of the results is discussed.


Item Type:Article
Additional Information:Copyright, 1945, by The Rockefeller Institute for Medical Research New York (Received for publication, April 2, 1945) This investigation was carried out with the aid of a grant from The Rockefeller Foundation. The author is indebted to Professor Linus Pauling, Dr. Verner Schomaker, Dr. Dan H. Campbell, and other associates for suggestions and discussion. Contribution No. 1004 fFrom the Gates and Crellin Laboratories of Chemistry, California Institute of Technology, Pasadena
Record Number:CaltechAUTHORS:WRIjem45
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:WRIjem45
Alternative URL:http://www.jem.org/cgi/content/abstract/81/6/647
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:4415
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:22 Aug 2006
Last Modified:26 Dec 2012 08:59

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