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The growth of bacteriophage and lysis of the host

Delbrück, M. (1940) The growth of bacteriophage and lysis of the host. Journal of General Physiology, 23 (5). pp. 643-660. ISSN 0022-1295. http://resolver.caltech.edu/CaltechAUTHORS:DELjgp40b

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Abstract

1. A new strain of B. coli and of phage active against it is described, and the relation between phage growth and lysis has been studied. It has been found that the phage can lyse these bacteria in two distinct ways, which have been designated lysis from within and lysis from without. 2. Lysis from within is caused by infection of a bacterium by a single phage particle and multiplication of this particle up to a threshold value. The cell contents are then liberated into solution without deformation of the cell wall. 3. Lysis from without is caused by adsorption of phage above a threshold value. The cell contents are liberated by a distension and destruction of the cell wall. The adsorbed phage is not retrieved upon lysis. No new phage is formed. 4. The maximum yield of phage in a lysis from within is equal to the adsorption capacity. 5. Liberation of phage from a culture in which the bacteria have been singly infected proceeds at a constant rate, after the lapse of a minimum latent period, until all the infected bacteria are lysed. 6. If the bacteria are originally not highly in excess, this liberation is soon counterbalanced by multiple adsorption of the liberated phage to bacteria that are already infected. This leads to a reduction of the final yield.


Item Type:Article
Additional Information:Copyright © 1940 by The Rockefeller University Press (Received for publication, January 29, 1940) The author wishes to express his appreciation for the hospitality extended to him by the Biology Department of the California Institute of Technology during the tenure of a Fellowship of The Rockefeller Foundation. In particular he wishes to record his indebtedness to Dr. E. L. Ellis for constant help and advice and to Mr. F. Gardner for technical assistance. Fellow of The Rockefeller Foundation
Record Number:CaltechAUTHORS:DELjgp40b
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:DELjgp40b
Alternative URL:http://www.jgp.org/cgi/content/abstract/23/5/643
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:4421
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:22 Aug 2006
Last Modified:26 Dec 2012 08:59

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