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Homeostatic Adjustment and Metabolic Remodeling in Glucose-limited Yeast Cultures

Brauer, Matthew J. and Saldanha, Alok J. and Dolinski, Kara and Botstein, David (2005) Homeostatic Adjustment and Metabolic Remodeling in Glucose-limited Yeast Cultures. Molecular and Cellular Biology, 16 (5). pp. 2503-2517. ISSN 0270-7306. http://resolver.caltech.edu/CaltechAUTHORS:BRAmbc05

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Abstract

We studied the physiological response to glucose limitation in batch and steady-state (chemostat) cultures of Saccharomyces cerevisiae by following global patterns of gene expression. Glucose-limited batch cultures of yeast go through two sequential exponential growth phases, beginning with a largely fermentative phase, followed by an essentially completely aerobic use of residual glucose and evolved ethanol. Judging from the patterns of gene expression, the state of the cells growing at steady state in glucose-limited chemostats corresponds most closely with the state of cells in batch cultures just before they undergo this "diauxic shift." Essentially the same pattern was found between chemostats having a fivefold difference in steady-state growth rate (the lower rate approximating that of the second phase respiratory growth rate in batch cultures). Although in both cases the cells in the chemostat consumed most of the glucose, in neither case did they seem to be metabolizing it primarily through respiration. Although there was some indication of a modest oxidative stress response, the chemostat cultures did not exhibit the massive environmental stress response associated with starvation that also is observed, at least in part, during the diauxic shift in batch cultures. We conclude that despite the theoretical possibility of a switch to fully aerobic metabolism of glucose in the chemostat under conditions of glucose scarcity, homeostatic mechanisms are able to carry out metabolic adjustment as if fermentation of the glucose is the preferred option until the glucose is entirely depleted. These results suggest that some aspect of actual starvation, possibly a component of the stress response, may be required for triggering the metabolic remodeling associated with the diauxic shift.


Item Type:Article
Additional Information:Copyright © 2005 by The American Society for Cell Biology. Submitted November 5, 2004; Accepted February 25, 2005. Originally published as MBC in Press, 10.1091/mbc.E04-11-0968 on March 9, 2005 For valuable comments, criticism, and suggestions, we thank Marian Carlson, Mark Johnston, Fred Winston, Maitreya Dunham, Pat Brown, and an anonymous reviewer. We also thank Evan Hurowitz for providing reverse transcription-PCR primer and probe sequences. This work was funded in part by grants from the National Institutes of Health to D. B. (GM-046406 and GM-071508) and to M.J.B. (HG-002649-01).
Record Number:CaltechAUTHORS:BRAmbc05
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:BRAmbc05
Alternative URL:http://dx.doi.org/10.1091/mbc.E04-11-0968
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:4574
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:29 Aug 2006
Last Modified:26 Dec 2012 09:00

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