CaltechAUTHORS
  A Caltech Library Service

The structure of a far-red fluorescent protein, AQ143, shows evidence in support of reported red-shifting chromophore interactions

Wannier, Timothy M. and Mayo, Stephen L. (2014) The structure of a far-red fluorescent protein, AQ143, shows evidence in support of reported red-shifting chromophore interactions. Protein Science, 23 (8). pp. 1148-1153. ISSN 0961-8368. PMCID PMC4116662. http://resolver.caltech.edu/CaltechAUTHORS:20140610-104728713

[img]
Preview
PDF - Accepted Version
See Usage Policy.

1316Kb

Use this Persistent URL to link to this item: http://resolver.caltech.edu/CaltechAUTHORS:20140610-104728713

Abstract

Engineering fluorescent proteins (FPs) to emit light at longer wavelengths is a significant focus in the development of the next generation of fluorescent biomarkers, as far-red light penetrates tissue with minimal absorption, allowing better imaging inside of biological hosts. Structure-guided design and directed evolution have led to the discovery of red FPs with significant bathochromic shifts to their emission. Here, we present the crystal structure of one of the most bathochromically shifted FPs reported to date, AQ143, a nine-point mutant of aeCP597, a chromoprotein from Actinia equina. The 2.19 Å resolution structure reveals several important chromophore interactions that contribute to the protein's far-red emission and shows dual occupancy of the green and red chromophores.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://onlinelibrary.wiley.com/doi/10.1002/pro.2498/abstractPublisherArticle
http://dx.doi.org/10.1002/pro.2498DOIArticle
http://onlinelibrary.wiley.com/doi/10.1002/pro.2498/suppinfoPublisherSupporting Information
Additional Information:© 2014 The Protein Society. Received: Apr 04, 2014; Revised: May 28, 2014; Accepted: May 29, 2014. Published online 3 June 2014 proteinscience.org The authors are grateful for the use of beamline 12-2 at the Stanford Synchrotron Radiation Lightsource (SSRL) in Menlo Park, CA, operated by Stanford University and supported by the Department of Energy and the National Institutes of Health. They additionally are thankful to Jens Kaiser and Pavle Nikolovski at the California Institute of Technology for helpful discussions. Finally they thank the Gordon and Betty Moore Foundation, the Beckman Institute, and the Sanofi-Aventis Bioengineering Research Program for support of the Molecular Observatory at the California Institute of Technology.
Funders:
Funding AgencyGrant Number
Department of Energy (DOE)UNSPECIFIED
NIHUNSPECIFIED
Gordon and Betty Moore FoundationUNSPECIFIED
Beckman InstituteUNSPECIFIED
Sanofi-Aventis Bioengineering Research ProgramUNSPECIFIED
Subject Keywords:Red Fluorescent Protein (RFP), Near-Infrared, Bathochromic Shift, Fluorescent Protein, Chromoprotein
PubMed Central ID:PMC4116662
Record Number:CaltechAUTHORS:20140610-104728713
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20140610-104728713
Official Citation:Wannier, T. M. and Mayo, S. L. (2014), The structure of a far-red fluorescent protein, AQ143, shows evidence in support of reported red-shifting chromophore interactions. Protein Science, 23: 1148–1153. doi: 10.1002/pro.2498
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:46177
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:10 Jun 2014 18:04
Last Modified:29 Aug 2014 20:30

Repository Staff Only: item control page