Todo, Takeshi and Roark, Margaret and Raghavan, K. Vijay and Mayeda, Carol and Meyerowitz, Elliot (1990) Fine-structure mutational analysis of a stage- and tissue-specific promoter element of the Drosophila glue gene Sgs-3. Molecular and Cellular Biology, 10 (11). pp. 5991-6002. ISSN 0270-7306 http://resolver.caltech.edu/CaltechAUTHORS:TODmcb90
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The Sgs-3 gene of Drosophila melanogaster exhibits a tightly regulated pattern of expression governed by two functionally equivalent elements within 1 kb of the gene, each of which is sufficient to confer third-instar salivary gland-specific transcription. In this report we describe a detailed functional analysis of one of these, the proximal element. To determine the nucleotides responsible for specific expression, we have introduced mutations into the proximal element and then assessed the effects of each alteration on expression in the developing animal. We have identified six particularly important base pairs which are located in two regions separated by nonessential sequences. These base pairs, along with some surrounding sequence, are conserved within the upstream regions of the three glue genes at 68C. Nearly identical groups of base pairs can be found upstream of the other glue genes which have been cloned. This analysis has allowed us to derive a consensus sequence, which we believe contains binding sites for two different factors which interact to direct third-instar salivary gland-specific expression.
|Additional Information:||Copyright © 1990 by the American Society for Microbiology. Received 29 May 1990;accepted 15 August 1990. The order of the first three authors on the title page is arbitrary. We thank Horst Kress for generously sharing data with us prior to publication. We are grateful to Mark D. Garfinkel, Annemarie Hofmann, and Peter H. Mathers for helpful discussions during the course of this work and to Bruce A. Hamilton and Michael J. Palazzolo for critical reading of the manuscript. This work was supported in part by a Public Health Service grant (GM28075) to E.M.M. from the Institute for General Medical Sciences, National Institutes of Health, and by a generous grant from the Lucille P. Markey Charitable Trust. T.T. was supported by a grant from the Procter and Gamble Co., and M.R. was supported by a National Research Service Award (GM 10694).|
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|Deposited By:||Lindsay Cleary|
|Deposited On:||01 Sep 2006|
|Last Modified:||26 Dec 2012 09:00|
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