Mitchell, Mary B. (1955) Aberrant Recombination of Pyridoxine Mutants of Neurospora. Proceedings of the National Academy of Sciences of the United States of America, 41 (4). pp. 215-220. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:MITpnas55a
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Giles  has reported that crosses between certain inositol mutants of Neurospora give infrequent wild recombinants, and from the segregation of linked markers it appeared that multiple crossovers might sometimes be involved. The present author subsequently observed that crosses between two closely linked or allelic pyrimidine mutants, one of which carried a "colonial" marker, gave occasional genetically wild offspring, but roughly half of these carried the "colonial" marker and half did not. This was surprising, since the marker appeared to be located only two or three units from the pyrimidine locus or loci, and, if the wild recombinants arose from crossovers between the two pyrimidine mutants, all would be expected to be alike with respect to the marker unless, in half the cases, a second crossover occurred between the pyrimidine locus and the locus of "colonial." These observations will be described in detail elsewhere. Unfortunately, a closely linked marker on the other side of pyrimidine is not known, and, moreover, crosses between these mutants are regularly semisterile, so that it is not possible to observe complete asci. However, another pair of mutants, requiring pyridoxine, also closely linked to "colonial" was found by Dr. T. H. Pittenger (personal communication) to exhibit the same behavior, and these mutants give fertile crosses. When a suitable marker was located  on the other side of pyridoxine, a study of recombinants among random spores and in complete asci was undertaken.
|Additional Information:||Copyright © 1955 by the National Academy of Sciences Communicated by G. W. Beadle, February 1, 1955 The author gratefully acknowledges the stimulating and most helpful interest with which Drs. H. K. Mitchell and David R. Stadler have followed this work. This work was supported by funds from the Atomic Energy Commission.|
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