Kallman, Frances and Williams, Robley C. and Dulbecco, Renato and Vogt, Marguerite (1958) Fine Structure of Changes Produced in Cultured Cells Sampled at Specified Intervals During a Single Growth Cycle of Polio Virus. Journal of Biophysical and Biochemical Cytology, 4 (3). pp. 301-308. http://resolver.caltech.edu/CaltechAUTHORS:KALjbbc58
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Primary suspended cultures of rhesus monkey kidney cells were infected with poliomyelitis virus, type 1 (Brunhilde strain). The release of virus from these cells over a one-step growth curve was correlated with their change in fine structure, as seen in the electron microscope. Most of the cells were infected nearly simultaneously, and morphological changes developed in the cells were sufficiently synchronous to be classified into three stages. The earliest change (stage I) became visible at a time when virus release into the culture fluid begins, some 3 hours after adsorption. Accentuation of the abnormal characteristics soon occurs, at 4 to 7 hours after adsorption, and results in stage II. Stage III represents the appearance of cells after their rate of virus release had passed its maximum, and probably the abnormal morphology of these cells reflects non-specific physiological damage. There seems to be consistency between the previously described cellular changes as seen under the light microscope and the finer scale changes reported here. Cytoplasmic bodies, called U bodies, were seen in large number at the time when the virus release was the most rapid (stage II). While these bodies are not of proper size to be considered polio virus, they seem to be specifically related to the infection. No evidence was found for the presence of particles that could even be presumptively identified with those of polio virus.
|Additional Information:||Copyright © 1958 by Rockefeller University Press (Received for publication, January 28, 1958) Aided by Grant C-2245 from the National Cancer Institute, National Institutes of Health, by Institutional Grant 43 H to the University of California from the American Cancer Society, and by a grant from the National Foundation for Infantile Paralysis. The authors wish to thank Mr. Joseph Toby for his able assistance in many of the procedures involving sectioning, microscopy, and photography. Includes PLATES 167 TO 172|
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|Deposited On:||03 Sep 2006|
|Last Modified:||26 Dec 2012 09:00|
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