Wu, Jung-Rung and Pearson, William R. and Posakony, James W. and Bonner, James (1977) Sequence Relationship between Long and Short Repetitive DNA of the Rat: A Preliminary Report. Proceedings of the National Academy of Sciences of the United States of America, 74 (10). pp. 4382-4386. ISSN 0027-8424 http://resolver.caltech.edu/CaltechAUTHORS:WUJpnas77
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Long and short repetitive sequences of rat DNA can be isolated and characterized. Long [>1.5 kilobases (kb)] sequences can be separated from short (0.2-0.4 kb) sequences by exclusion chromatography after renaturation of 4-kb DNA fragments to a repetitive C0t and digestion with the single-strand-specific S1 nuclease. (C0t is the initial concentration of DNA in mol of nucleotides/liter multiplied by time in sec.) Long repetitive DNA can be driven by an excess of whole rat DNA to measure its repetitive frequency. Excess long repetitive DNA can also be used to drive tracer quantities of either long (self-renaturation) or short repetitive DNA. Both the extent and the rate of the renaturations are found to be similar, suggesting that long and short DNA fragments share sequences. When long repetitive DNA is used to drive whole DNA tracers of various lengths, a 3.2-kb interspersion period is found. These data are consistent with the concept that short repetitive sequences are present within long repetitive DNA sequences in the rat genome.
|Additional Information:||Copyright © 1977 by the National Academy of Sciences Contributed by James Bonner, August 8, 1977 We thank Dr. Francine Eden and Ms. Denise Painchaud for the gift of the SI nuclease used in this study, Dr. Anthony Bakke for valuable discussion, and Mr. Bill Buchanan for his technical assistance. This research was supported in part by U.S. Public Health Service Training Grant GM00086 and in part by U.S. Public Health Service Research Grants GM13762 and GM20927. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U. S. C. §1734 solely to indicate this fact.|
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