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Mechanism of leukemogenesis induced by mink cell focus-forming murine leukemia viruses

Li, Jing-Po and Baltimore, David (1991) Mechanism of leukemogenesis induced by mink cell focus-forming murine leukemia viruses. Journal of Virology, 65 (5). pp. 2408-2414. ISSN 0022-538X. http://resolver.caltech.edu/CaltechAUTHORS:LIVjvir91

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Abstract

The Friend or Moloney mink cell focus-forming (MCF) virus encodes a recombinant-type envelope glycoprotein, gp 70, that is closely related to the membrane glycoprotein, gp55, of Friend spleen focus-forming virus (SFFV). We have shown previously that gp55 has the ability to activate cell growth by binding to the cellular receptor for erythropoietin. Here we show that gp70 encoded by either the Friend of Moloney MCF virus also binds to the erythropoietin receptor and that coexpression of the receptor and gp70 in an interleukin-3 (IL-3)-dependent cell line can activate IL-3-independent growth. Furthermore, when the cDNA for the human IL-2 receptor beta chain, which is related by sequence to the erythropoietin receptor, was introduced into this cell line, it became growth factor independent after infection either with SFFV or with one of the two MCF viruses but not with an ecotropic virus. Based on these observations, we propose a mechanism for the early stage of luekemogenesis induced by the MCF-type murine leukemia viruses.


Item Type:Article
Additional Information:Copyright © 1991 American Society for Microbiology. Received 31 October 1990; accepted 28 January 1991. We thank A. Ishimoto and I. Verma for providing the molecular clones for F-MCF and M-MCF viruses, respectively, and D. Kabat for providing the R-MuLV-producing Rwt cells. We also thank T. Taniguchi for providing the plasmid pIL-2Rβ30, M. Tsudo for providing anti-human IL-2RP monoclonal antibody (Mik-β1), A. D'Andrea and H. Lodish for providing an anti-Epo-R antiserum, and S. Ruscetti for providing 7C10 monoclonal antibody. We are grateful to A. D'Andrea and H. Lodish for helpful discussions. This work was supported by Public Health Service grant AI 22346 to D.B. from the National Institutes of Health.
Subject Keywords:LONG TERMINAL REPEAT; RECEPTOR-BETA-CHAIN; COMPLETE NUCLEOTIDE-SEQUENCE; ENV GENE; MEMBRANE GLYCOPROTEIN; ENVELOPE GENE; PROVIRAL DNA; FRIEND-VIRUS; 3' END; NONDEFECTIVE FRIEND
Record Number:CaltechAUTHORS:LIVjvir91
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:LIVjvir91
Alternative URL:http://jvi.asm.org/cgi/content/abstract/65/5/2408
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:4831
Collection:CaltechAUTHORS
Deposited By: Lindsay Cleary
Deposited On:11 Sep 2006
Last Modified:26 Dec 2012 09:01

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