Umeno, Daisuke and Arnold, Frances H. (2004) Evolution of a Pathway to Novel Long-Chain Carotenoids. Journal of Bacteriology, 186 (5). pp. 1531-1536. ISSN 0021-9193. http://resolver.caltech.edu/CaltechAUTHORS:UMEjbact04
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Using methods of laboratory evolution to force the C30 carotenoid synthase CrtM to function as a C40 synthase, followed by further mutagenesis at functionally important amino acid residues, we have discovered that synthase specificity is controlled at the second (rearrangement) step of the two-step reaction. We used this information to engineer CrtM variants that can synthesize previously unknown C45 and C50 carotenoid backbones (mono- and diisopentenylphytoenes) from the appropriate isoprenyldiphosphate precursors. With this ability to produce new backbones in Escherichia coli comes the potential to generate whole series of novel carotenoids by using carotenoid-modifying enzymes, including desaturases, cyclases, hydroxylases, and dioxygenases, from naturally occurring pathways.
|Additional Information:||Copyright © 2004, American Society for Microbiology. Received 5 June 2003/ Accepted 30 October 2003 This research was supported by the U.S. National Science Foundation (BES-0118565) and Maxygen, Inc.|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Archive Administrator|
|Deposited On:||11 Sep 2006|
|Last Modified:||26 Dec 2012 09:01|
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