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Studies on Nucleic Acid Reassociation Kinetics: Rate of Hybridization of Excess RNA with DNA, Compared to the Rate of DNA Renaturation

Galau, Glenn A. and Britten, Roy J. and Davidson, Eric H. (1977) Studies on Nucleic Acid Reassociation Kinetics: Rate of Hybridization of Excess RNA with DNA, Compared to the Rate of DNA Renaturation. Proceedings of the National Academy of Sciences of the United States of America, 74 (3). pp. 1020-1023. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:GALpnas77a

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Abstract

The rate of reaction of double-stranded replicative form (RF)[3H]DNA of bacteriophage phi X174 with excess (+)strand DNA and (+)strand RNA was measured by standard methods of hydroxyapatite chromatography. The reactions follow pseudo-first-order kinetics and the observed rate constant for the RNA· DNA reaction differs less than 25% from that of the DNA· DNA reaction. The pseudo-first-order rate constants are close to the value predicted on the basis of the second-order rate constant measured in the renaturation of the double-stranded phi X RF [3H]DNA.


Item Type:Article
Additional Information:Copyright © 1977 by the National Academy of Sciences Contributed by Roy J. Britten, January 10, 1977 We gratefully acknowledge the gifts of φX174 nucleic acids by Drs. Lloyd Smith, Amy S. Lee, Paul H. Johnson, and Robert L. Sinsheimer. We thank Dr. Norman Davidson for his critical review of the manuscript. This research was supported by National Institutes of Health Grants HD-05753 and GM-20927 and by National Science Foundation Grant BMS 75-07359. This is paper III in a series. Paper II is ref. 15.
Subject Keywords:bacteriophage φX174 nucleic acids; hydroxyapatite chromatography
Record Number:CaltechAUTHORS:GALpnas77a
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:GALpnas77a
Alternative URL:http://www.pnas.org/cgi/content/abstract/74/3/1020
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:4906
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:12 Sep 2006
Last Modified:26 Dec 2012 09:02

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