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Caenorhabditis elegans Inositol 5-Phosphatase Homolog Negatively Regulates Inositol 1,4,5-Triphosphate Signaling in Ovulation

Bui, Yen Kim and Sternberg, Paul W. (2002) Caenorhabditis elegans Inositol 5-Phosphatase Homolog Negatively Regulates Inositol 1,4,5-Triphosphate Signaling in Ovulation. Molecular Biology of the Cell, 13 (5). pp. 1641-1651. ISSN 1059-1524. PMCID PMC111133. http://resolver.caltech.edu/CaltechAUTHORS:BUImbc02

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Abstract

Ovulation in Caenorhabditis elegans requires inositol 1,4,5-triphosphate (IP3) signaling activated by the epidermal growth factor (EGF)-receptor homolog LET-23. We generated a deletion mutant of a type I 5-phosphatase, ipp-5, and found a novel ovulation phenotype whereby the spermatheca hyperextends to engulf two oocytes per ovulation cycle. The temporal and spatial expression of IPP-5 is consistent with its proposed inhibition of IP3 signaling in the adult spermatheca. ipp-5 acts downstream of let-23, and interacts with let-23-mediated IP3 signaling pathway genes. We infer that IPP-5 negatively regulates IP3 signaling to ensure proper spermathecal contraction.


Item Type:Article
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC111133/PubMed CentralArticle
ORCID:
AuthorORCID
Sternberg, Paul W.0000-0002-7699-0173
Additional Information:Copyright © 2002 by The American Society for Cell Biology. Under the License and Publishing Agreement, authors grant to the general public, effective two months after publication of (i.e.,. the appearance of) the edited manuscript in an online issue of MBoC, the nonexclusive right to copy, distribute, or display the manuscript subject to the terms of the Creative Commons–Noncommercial–Share Alike 3.0 Unported license (http://creativecommons.org/licenses/by-nc-sa/3.0). Submitted October 3, 2001; Revised January 18, 2002; Accepted February 1, 2002. Monitoring Editor: Judith Kimble. Originally published as MBC in Press, 10.1091/mbc.02-01-0008 on April 3, 2002 We thank Y. Hadju-Cronin, J. Copeland, and B. Bingol for help isolating sy605; Y. Kohara for cDNA; J. Thomas for sa62 and sa73; A. Fire for GFP and HS vectors; A. Parker for the pCeh promoter and strain KR3738; A. Hart for the nlp-8 promoter and strain PT4; L.R. Garcia, E. Schwarz, other members of our laboratory, and an anonymous reviewer for valuable discussion and comments on the manuscript; and L. Maxfield (Caltech Digital Media Center) for help making web movies. The Caenorhabditis Genetics Center provided some strains. This project was supported by the Howard Hughes Medical Institute, with which P.W.S is an investigator. Y.K.B. is a National Institutes of Health trainee supported by National Institutes of Health grant 5T32GM07737.
PubMed Central ID:PMC111133
Record Number:CaltechAUTHORS:BUImbc02
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:BUImbc02
Alternative URL:http://dx.doi.org/10.1091/mbc.02-01-0008
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:5003
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:18 Sep 2006
Last Modified:22 Sep 2015 17:25

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