Table 1.  ipp-5(sy605) suppresses the sterility of lin-3(rf) and let-23(rf) and synergizes with other genes in the let-23-mediated fertility pathway

Genotype
other ipp-5 Fertile n Brood

+ +/+ 100% 20 337  33
let-23(rf) +/+ 0% 40 0
lin-3(rf) +/+ 1.3% 76 0.2  1.8
+ sy605/sy605 100% 25 144  20
+ sy605/+ 100% 20 314  36
let-23(rf) sy605/sy605 100% 21 n.d.a
lin-3(rf) sy605/sy605 98.7% 319 28  9 (n = 47)
lfe-1(gf) +/+ 100% 26 207  35
lfe-2(lf) +/+ 100% 23 213  34
lfe-1(gf) sy605/sy605 24% 55 6.0  12
lfe-2(lf) sy605/sy605 0% 86 0

Fertile is defined as having greater than five offspring. Alleles used are lin-3(n1058), let-23(sy10), lfe-1(sy290) and lfe-2(sy326). unc-4(e120) was used as a marker and mnC1[dpy-10(e128) unc-52(e444)] as a balancer for let-23, and unc-24(e138) was used as a marker and DnT1[nT1[unc(n745dm)let] as a balancer for lin-3. lfe-1 was marked with unc-24(e138) and lfe-2 was marked with unc-57(ad592).
a  sy10 animals display a partially penetrant larval lethal phenotype associated with let-23(rf) decreases in RAS signaling, thus broods were not determined. n.d., not determined.