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Carbonate-hosted methanotrophy represents an unrecognized methane sink in the deep sea

Marlow, Jeffrey J. and Steele, Joshua A. and Ziebis, Wiebke and Thurber, Andrew R. and Levin, Lisa A. and Orphan, Victoria J. (2014) Carbonate-hosted methanotrophy represents an unrecognized methane sink in the deep sea. Nature Communications, 5 (10). Art. No. 6094. ISSN 2041-1723. http://resolver.caltech.edu/CaltechAUTHORS:20141002-104139439

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Abstract

The atmospheric flux of methane from the oceans is largely mitigated through microbially mediated sulphate-coupled methane oxidation, resulting in the precipitation of authigenic carbonates. Deep-sea carbonates are common around active and palaeo-methane seepage, and have primarily been viewed as passive recorders of methane oxidation; their role as active and unique microbial habitats capable of continued methane consumption has not been examined. Here we show that seep-associated carbonates harbour active microbial communities, serving as dynamic methane sinks. Microbial aggregate abundance within the carbonate interior exceeds that of seep sediments, and molecular diversity surveys reveal methanotrophic communities within protolithic nodules and well-lithified carbonate pavements. Aggregations of microbial cells within the carbonate matrix actively oxidize methane as indicated by stable isotope FISH–nanoSIMS experiments and ^(14)CH_4 radiotracer rate measurements. Carbonate-hosted methanotrophy extends the known ecological niche of these important methane consumers and represents a previously unrecognized methane sink that warrants consideration in global methane budgets.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1038/ncomms6094DOIArticle
http://www.nature.com/ncomms/2014/141014/ncomms6094/abs/ncomms6094.htmlPublisherArticle
http://www.nature.com/ncomms/2014/141014/ncomms6094/extref/ncomms6094-s1.pdfPublisherSupplemental Material
http://www.nature.com/ncomms/2014/141014/ncomms6094/extref/ncomms6094-s2.xlsxPublisherSupplemental Material
http://www.nature.com/ncomms/2014/141014/ncomms6094/extref/ncomms6094-s3.xlsxPublisherSupplemental Material
http://www.nature.com/ncomms/2014/141014/ncomms6094/extref/ncomms6094-s4.xlsxPublisherSupplemental Material
http://rdcu.be/cmnLPublisherFree ReadCube access
ORCID:
AuthorORCID
Orphan, Victoria J.0000-0002-5374-6178
Additional Information:© 2014 Macmillan Publishers Limited. Received 24 March 2014; Accepted 28 August 2014; Published 14 October 2014. We thank the Captains, Crew, Alvin group, Jason group and Science party members from RV Atlantis legs AT-15-11, AT-15-44, AT-15-59, AT-15-68 and AT-18-10. Patricia Tavormina and Stephanie Connon kindly helped with pmo analysis and molecular cloning procedures, and Olivia Mason prepared the 16S rRNA gene clone library sequences for A.Nod-2518. Anne Dekas, Abigail Green-Saxena and Yunbin Guan aided with nanoSIMS operation, and Alex Parker assisted with porosity and permeability measurements. Shawn McGlynn, Roland Hatzenpichler, Jennifer Glass, Kat Dawson, Hank Yu, David Case and Hiroyuki Imachi provided useful comments throughout the experimentation and writing process. This study was funded by grants from the National Science Foundation (OCE-0825791 and OCE-0939559), the National Aeronautics and Space Administration (NASA) Astrobiology Institute (award #NNA04CC06A and #NNA13AA92A) and the Gordon and Betty Moore Foundation Marine Microbiology Initiative Grant #3780, to V.J.O. J.J.M. was partially supported by a National Energy Technology Laboratory Methane Hydrate Research Fellowship funded by the National Research Council of the National Academies. NCBI Reference Sequence: KF616507; KF616827
Funders:
Funding AgencyGrant Number
NSFOCE-0825791
NSFOCE-0939559
NASANNA04CC06A
NASANNA13AA92A
Gordon and Betty Moore Foundation3780
National Research CouncilUNSPECIFIED
Record Number:CaltechAUTHORS:20141002-104139439
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20141002-104139439
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:50167
Collection:CaltechAUTHORS
Deposited By: Joy Painter
Deposited On:14 Oct 2014 18:11
Last Modified:09 Jan 2016 02:51

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