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Requirement for the NF-kappa B family member Re1A in the development of secondary lymphoid organs

Alcamo, Elizabeth and Hacohen, Nir and Schulte, Leah C. and Rennert, Paul D. and Hynes, Richard O. and Baltimore, David (2002) Requirement for the NF-kappa B family member Re1A in the development of secondary lymphoid organs. Journal of Experimental Medicine, 195 (2). pp. 233-244. ISSN 0022-1007. http://resolver.caltech.edu/CaltechAUTHORS:ALCjem02

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Abstract

The transcription factor nuclear factor (NF)-kappaB has been suggested to be a key mediator of the development of lymph nodes and Peyer's patches. However, targeted deletion of NF-kappaB/ Rel family members has not yet corroborated such a function. Here we report that when mice lacking the RelA subunit of NF-kappaB are brought to term by breeding onto a tumor necrosis factor receptor (TNFR)1-deficient background, the trice that are born lack lymph nodes, foyer's patches, and an organized splenic microarchitecture, and have a profound defect in T cell-dependent antigen responses. Analyses of TNFR1/1RelA-deficient embryonic tissues and of radiation chimeras suggest that the dependence on RelA is manifest not in hematopoietic cells but rather in radioresistant stromal cells needed for the development of secondary lymphoid organs.


Item Type:Article
Additional Information:©2002 Rockefeller University Press. Submitted: November 13, 2001; accepted: November 30, 2001. We wish to thank Jeffrey L. Browning and colleagues at Biogen, Inc. for creating, characterizing, and producing LT-specific reagents; Denise Crowley for advice on immunohistochemistry; Jianzhu Chen for advice on antibody analysis; Paul Majewski for technical assistance; and Stephen Robinson for helpful suggestions and critical review of this work as it progressed. This work was supported by the National Institutes of Health grants (to D. Baltimore and E. Alcamo), and by grants from the Whitehead Institute Fellows Program, Hascoe Foundation, and Rippel Foundation (to N. Hacohen).
Subject Keywords:lymph nodes; Peyer's patches; spleen; p65; TNFR1
Record Number:CaltechAUTHORS:ALCjem02
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:ALCjem02
Alternative URL:http://dx.doi.org/10.1084/jem.20011885
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:5111
Collection:CaltechAUTHORS
Deposited By: Lindsay Cleary
Deposited On:02 Oct 2006
Last Modified:26 Dec 2012 09:03

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