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Regulation of Murine Class I Genes by Interferons is Controlled by Regions Located Both 5' and 3' to the Transcription Initiation Site

Korber, Bette and Hood, Leroy and Stroynowski, Iwona (1987) Regulation of Murine Class I Genes by Interferons is Controlled by Regions Located Both 5' and 3' to the Transcription Initiation Site. Proceedings of the National Academy of Sciences of the United States of America, 84 (10). pp. 3380-3384. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:KORpnas87

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Abstract

Interferons regulate the expression of a large number of mammalian genes, including the major histocompatibility antigen genes. To investigate the mechanisms involved in interferon action, we have analyzed the ability of murine H-2Ld and H-2Dd DNA sequences to control the responses to interferon. The results indicate that interferon regulation of class I gene expression is complex and involves at least two mechanisms that are dependent on class I sequences located upstream and downstream to the transcription initiation site. In transfected mouse L cells, both of these regions are required for full enhancement of class I gene expression, with the major portion of the response controlled by the sequences located 3' to the transcription initiation site. The fine-mapping analysis of the 5' region-encoded response also suggests that recombinant alpha and gamma interferons may exert their effects on class I gene expression by using different cis-acting regulatory sequences.


Item Type:Article
Additional Information:Copyright © 1987 by the National Academy of Sciences Contributed by Leroy Hood, January 7, 1987 We thank Kay Blackburn for assistance with tissue culture, and Drs. U. Landegren and S. Hunt for many thoughtful conversations concerning the work. We thank Drs. A. Israel, L. Hoops, N. Lan, P. Lengyel, K. Ozato, S. Rosenberg, E. Rothenberg, and B. Wold for criticism of the manuscript. We are grateful to M. Shepard of Genentech for providing IFN-γ, P. Sorter of Hoffman-La Roche Inc. for IFN-α A/D, and T. Hansen and K. Ozato for monoclonal antibodies. This work was supported by National Research Service Award T32GM7616 and by National Institutes of Health Grant AI 19624. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Subject Keywords:interferon action; transplantation antigens; gene regulation
Record Number:CaltechAUTHORS:KORpnas87
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:KORpnas87
Alternative URL:http://www.pnas.org/cgi/content/abstract/84/10/3380
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:5175
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:04 Oct 2006
Last Modified:14 Nov 2014 19:19

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