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Recombinant DNA Molecules of Bacteriophage phi X174

Benbow, Robert M. and Zuccarelli, Anthony J. and Sinsheimer, Robert L. (1975) Recombinant DNA Molecules of Bacteriophage phi X174. Proceedings of the National Academy of Sciences of the United States of America, 72 (1). pp. 235-239. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:BENpnas75

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Abstract

phi X174 DNA structures containing two different parental genomes were detected genetically and examined by electron microscopy. These structures consisted of two monomeric double-stranded DNA molecules linked in a figure 8 configuration. Such DNA structures were observed to be formed preferentially in host recA+ cells or recA+ cell-free systems. Since the host recA+ allele is required for most phi X174 recombinant formation, we conclude that the observed figure 8 molecules are intermediates in, or end products of, a phi X174 recombination event. We propose that recombinant figure 8 DNA molecules arise as a result of "single-strand aggression," are stabilized by double-strand "branch migration," and represent a specific example of a common intermediate in genetic recombination.


Item Type:Article
Additional Information:Copyright © 1975 by the National Academy of Sciences Contributed by Robert L. Sinsheimer, March 16, 1974 An oral account of this work was given to the Genetic Recombination Symposium, Bethesda, Md., 6-9 June, 1972, to the British Genetical Society, Kent, April 1973, and to the European Molecular Biology Organization meeting at Aviemore, England, June 1973. This research was supported in part by Grant GM 13554 from the U.S. Public Health Service.
Subject Keywords:electron microscopy; spheroplast assay; multiple length DNA molecules; recombination models
Record Number:CaltechAUTHORS:BENpnas75
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:BENpnas75
Alternative URL:http://www.pnas.org/cgi/content/abstract/72/1/235
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:5223
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:05 Oct 2006
Last Modified:26 Dec 2012 09:04

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