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Deduced Primary Structure of the β Subunit of Brain Type II Ca2+/calmodulin-dependent Protein Kinase Determined by Molecular Cloning

Bennett, Mark K. and Kennedy, Mary B. (1987) Deduced Primary Structure of the β Subunit of Brain Type II Ca2+/calmodulin-dependent Protein Kinase Determined by Molecular Cloning. Proceedings of the National Academy of Sciences of the United States of America, 84 (7). pp. 1794-1798. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:BENpnas87

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Abstract

cDNA clones coding for the β subunit of rat brain type II Ca2+/calmodulin-dependent protein kinase were isolated and sequenced. The clones, including one containing the entire coding region, hybridize at high stringency to a single band of poly(A)+ RNA of length 4.8 kilobases. The subunit coded for by the clones was identified by in vitro transcription of the cDNA followed by translation of the resulting RNA. The DNA sequence of the clones contains a single long open reading frame (1626 nucleotides) coding for a protein of 542 amino acids with a molecular weight of 60,333, the amino-terminal half of which is homologous to several other protein kinases. Potential ATP- and calmodulin-binding domains were identified. Two independent clones contain an identical 45-nucleotide deletion, relative to the clones described above, resulting in a shorter open reading frame coding for a protein of molecular weight 58,000. This suggests that the minor, 58-kDa β' subunit of the type II Ca2+/calmodulin-dependent kinase may be synthesized on a separate message.


Item Type:Article
Additional Information:Copyright © 1987 by the National Academy of Sciences Communicated by John D. Roberts, November 18, 1986 We thank Dr. D. Anderson for the gift of the λ libraries and C. Hochenedel and J. White for help with preparation of the manuscript. This work was supported by National Institutes of Health Grants NS17660 and IT32 GM07616, the McKnight Foundation, the Pugh Foundation, and the Gustavus and Louise Pfeiffer Research Foundation. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Subject Keywords:protein phosphorylation; synaptic regulation
Record Number:CaltechAUTHORS:BENpnas87
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:BENpnas87
Alternative URL:http://www.pnas.org/cgi/content/abstract/84/7/1794
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:5224
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:05 Oct 2006
Last Modified:26 Dec 2012 09:04

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