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A Method for the Rapid Cultivation of Desulfovibrio aestuarii on Filter Membranes

Tsuneishi, N. and Goetz, A. (1958) A Method for the Rapid Cultivation of Desulfovibrio aestuarii on Filter Membranes. Applied Microbiology, 6 (1). pp. 42-44. ISSN 0003-6919. http://resolver.caltech.edu/CaltechAUTHORS:TSUappmicrob58

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Abstract

The cultivation of sulfate-reducing bacteria on agar plates is a somewhat involved and time-consuming process. This is partly due to the requirement for fairly strict anaerobic conditions but mainly because of the length of the incubation period which may vary from 6 days to weeks with the resulting delay of the required information. The work reported herein was undertaken in an attempt to apply the advantageous features of the membrane filter method to the detection of anaerobic organisms. As originally developed, the membrane filter (Goetz and Tsuneishi, 1951; Clark et al., 1951) permits the recovery of small numbers of organisms from large volumes of water and the rapid cultivation of many aerobic bacteria. The ultimate purpose was to develop a method which would not require more complex manipulation than the membrane procedure according to Standard Methods for the Examination of Water, Sewage, and Industrial Wastes (A.P.H.A. 1955) and to reduce the incubation period for anaerobic bacteria.


Item Type:Article
Additional Information:Copyright © 1958 American Society for Microbiology. Received for publication July 8, 1957 The authors wish to acknowledge the advice in the matter of nutrient composition received from Mr. H.F. Keller, Research Process Department of Union Company, Brea, California and from Mr. H. Wiener, Research Laboratory of Shell Oil Company, Los Angeles, California, as well as for supplying cultures of Desulfovibrio aestuarii. The senior author (A.G.) wishes to express his obligation for some stimulating discussions on the subject with Prof. Claude E. ZoBell, Scripps Institution of Oceanography, La Jolla, California. Presented at the 56th General MIeeting of the Society of American Bacteriologists, Houston, Texas, May 1956. Bacteriol. Proc. 1956, Soc. Am. Bacteriologists, p. 54.
Record Number:CaltechAUTHORS:TSUappmicrob58
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:TSUappmicrob58
Alternative URL:http://aem.asm.org/content/vol6/issue1/index.dtl
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ID Code:5389
Collection:CaltechAUTHORS
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Deposited On:16 Oct 2006
Last Modified:26 Dec 2012 09:05

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