Falkenthal, Scott and Parker, Vann P. and Mattox, William W. and Davidson, Norman (1984) Drosophila melanogaster has only one myosin alkali light-chain gene which encodes a protein with considerable amino acid sequence homology to chicken myosin alkali light chains. Molecular and Cellular Biology, 4 (5). pp. 956-965. ISSN 0270-7306 http://resolver.caltech.edu/CaltechAUTHORS:FALmcb84
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A chimeric lambda DNA molecule containing the myosin alkali light-chain gene of Drosophila melanogaster was isolated. The encoded amino acid sequence was determined from the nucleic acid sequence of a cDNA homologous to the genomic clone. The identity of the encoded protein was established by two criteria: (i) sequence homology with the chicken alkali light-chain proteins and (ii) comparison of the two-dimensional gel electrophoretic pattern of the peptides synthesized by in vitro translation of hybrid-selected RNA to that of myosin alkali light-chain peptides extracted from Drosophila myofibrils. There is only one myosin alkali light-chain in D. melanogaster; its chromosomal location is region 98B. This gene is abundantly expressed during the development of larval as well as adult muscles. The Drosophila protein appears to contain one putative divalent cation-binding domain (an EF hand) as compared with the three EF hands present in chicken alkali light chains.
|Additional Information:||Copyright © 1984 by the American Society for Microbiology. Received 9 January 1984/Accepted 27 February 1984 We thank E. Fyrberg and C. Rozek for their generous gifts of actin and myosin heavy-chain-coding DNAs and T. Hunkapiller for heli in the execution of the protein homology comparisons. We also thank H. Mitchell and N. Peterson for guidance and direction in pupal dissection and sharing their results of pupal indirect flight muscle development. S.F. was supported by a Muscular Dystrophy Postdoctoral Fellowship and NRSA Fellowship 1 F32 GM08895-01. The research was supported by Public Health Service grants from the National Institutes of Health.|
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